The technological landscape and applications of single-cell multi-omics
- PMID: 37280296
- PMCID: PMC10242609
- DOI: 10.1038/s41580-023-00615-w
The technological landscape and applications of single-cell multi-omics
Abstract
Single-cell multi-omics technologies and methods characterize cell states and activities by simultaneously integrating various single-modality omics methods that profile the transcriptome, genome, epigenome, epitranscriptome, proteome, metabolome and other (emerging) omics. Collectively, these methods are revolutionizing molecular cell biology research. In this comprehensive Review, we discuss established multi-omics technologies as well as cutting-edge and state-of-the-art methods in the field. We discuss how multi-omics technologies have been adapted and improved over the past decade using a framework characterized by optimization of throughput and resolution, modality integration, uniqueness and accuracy, and we also discuss multi-omics limitations. We highlight the impact that single-cell multi-omics technologies have had in cell lineage tracing, tissue-specific and cell-specific atlas production, tumour immunology and cancer genetics, and in mapping of cellular spatial information in fundamental and translational research. Finally, we discuss bioinformatics tools that have been developed to link different omics modalities and elucidate functionality through the use of better mathematical modelling and computational methods.
© 2023. Springer Nature Limited.
Conflict of interest statement
R.F. is scientific founder and adviser for IsoPlexis, Singleron Biotechnologies and AtlasXomics. The interests of R.F. were reviewed and managed by Yale University Provost’s Office in accordance with the University’s conflict of interest policies. In the past three years, R.S. has worked as a consultant for Bristol-Myers Squibb, Regeneron and Kallyope and served as an Scientific Advisory Board member for ImmunAI, Resolve Biosciences, Nanostring and the NYC Pandemic Response Lab. A.B. and Z.B. declare no competing interests.
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