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. 2023 Jan 10:13:1091090.
doi: 10.3389/fphar.2022.1091090. eCollection 2022.

The mechanism of oxymatrine on atopic dermatitis in mice based on SOCS1/JAK-STAT3 pathway

Xianwei Han  1 , Tianming Ma  2 , Qiang Wang  1 , Chunlin Jin  1 , Yusheng Han  3 , Guijun Liu  3 , Hao Li  4
Affiliations

The mechanism of oxymatrine on atopic dermatitis in mice based on SOCS1/JAK-STAT3 pathway

Xianwei Han et al. Front Pharmacol. .

Abstract

Based on the suppressor of cytokine signaling 1 (SOCS1)/Janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) pathway, the mechanism of oxymatrine in the treatment of atopic dermatitis (AD) was preliminarily explored in this study. C57BL/6 mice were induced to establish AD model by smearing carbotriol (MC903) on their back. The AD mice were randomly divided into model group, oxymatrine groups with three dosages (25, 50 and 100 mg/kg), (n = 10). Oxymatrine groups were intragastric administered once daily for 14 days. The same volume of saline was given in the normal control group and model group once daily for 14 days. Subsequently, HE staining was used to observe the pathological changes of skin tissue, ELISA was used to detect the levels of serum inflammatory factors including interleukin-4, 6 and 17 (IL-4, IL-6, and IL-17), tumor necrosis factor-α (TNF-α) and immunoglobulin E (IgE). Immunohistochemistry was used to detect the expression of suppressor of cytokine signaling 1 and CD3 in skin tissue, and Western blotting was used to detect the proteins in suppressor of cytokine signaling 1/JAK-STAT3 pathway. Compared with the normal control group, the pathological damage of mice in the model group, such as skin hyperplasia, edema, congestion and inflammatory infiltration, aggravated increased significantly. And the expression of serum inflammatory factors, CD3 positive expression and JAK-STAT3 pathway protein in the model group were increased (p < .05), and the expression of suppressor of cytokine signaling 1 protein (p < .05) was decreased. Compared with the model group, the above pathological damage of the mice was reduced, and the serum inflammatory factors, JAK-STAT3 pathway protein, and CD3 positive expression were decreased as a dose-dependant manner (p < .05), and the expression of suppressor of cytokine signaling 1 protein was increased as a dose-dependent manner (p < .05). Oxymatrine can improve the skin inflammation symptoms of AD mice by up regulating the expression of suppressor of cytokine signaling 1, inhibiting the activation of JAK-STAT3 pathway and blocking the activation of T lymphocytes.

Keywords: JAK-STAT3; SOCS1; atopic dermatitis; janus kinase; oxymatrine.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Histopathological changes of skin tissue (HE staining, × 200). (A) Control group; (B) Model group. (C) OMT-H group. (D) OMT-M group. (E) OMT-L group.
FIGURE 2
FIGURE 2
Comparison of (A) IgE, (B) IL-4, (C) IL-6, (D) IL-17 and (E) TNF-α levels in serum. (##, p < .01, vs. Control group; **, p < .01, vs. model group).
FIGURE 3
FIGURE 3
Positive expression of CD3 in skin tissue (immunohistochemical staining, × 200). (A) Normal control group; (B) Model group. (C) OMT-H group. (D) OMT-M group. (E) OMT-L group. (F) Statistical data of positive expression of CD3 in each group. (##, p < .01, vs. Control group; **, p < .01, vs. model group).
FIGURE 4
FIGURE 4
Positive expression of SOCS1 in skin tissue (immunohistochemical staining, × 200). (A) Normal control group; (B) Model group. (C) OMT-H group. (D) OMT-M group. (E) OMT-L group. (F) Statistical data of positive expression of SOCS1 in each group. (##, p < .01, vs. Control group; **, p < .01, vs. model group).
FIGURE 5
FIGURE 5
Expression of SOCS1 protein in skin tissue (β-actin image for control were the same in Figure 5 and Figure 6). (A) Normal control group; (B) Model group. (C) OMT-H group. (D) OMT-M group. (E) OMT-L group. (F) Statistical data of expression of SOCS1 protein in each group. (##, p < .01, vs. Control group; **, p < .01, vs. model group).
FIGURE 6
FIGURE 6
Expression of JAK1, p-JAK1, STAT3 and p-STAT3 protein in skin tissue (β-actin image for control were the same in Figure 5 and Figure 6). (A) Normal control group; (B) Model group. (C) OMT-H group. (D) OMT-M group. (E) OMT-L group. (F) Statistical data of expression of JAK1, p-JAK1, STAT3 and p-STAT3 protein in each group. (##, p < .01, vs. Control group; **, p < .01, vs. model group).
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Grants and funding

This study was supported by the Natural Science Foundation of Liaoning Province, China (2021-MS-368), the Fund of Public Health Research and Development Project of Shenyang Bureau of Science and Technology (20-205-4-037) and the Scientific Research Project of Health Commision of Shenyang, Liaoning Province (2021047).