Lack of tRNA modification isopentenyl-A37 alters mRNA decoding and causes metabolic deficiencies in fission yeast
- PMID: 23716598
- PMCID: PMC3719670
- DOI: 10.1128/MCB.00278-13
Lack of tRNA modification isopentenyl-A37 alters mRNA decoding and causes metabolic deficiencies in fission yeast
Erratum in
-
Erratum for Lamichhane et al., lack of tRNA modification isopentenyl-A37 alters mRNA decoding and causes metabolic deficiencies in fission yeast.Mol Cell Biol. 2015 Apr;35(8):1477. doi: 10.1128/MCB.00170-15. Mol Cell Biol. 2015. PMID: 25805774 Free PMC article. No abstract available.
Abstract
tRNA isopentenyltransferases (Tit1) modify tRNA position 37, adjacent to the anticodon, to N6-isopentenyladenosine (i6A37) in all cells, yet the tRNA subsets selected for modification vary among species, and their relevance to phenotypes is unknown. We examined the function of i6A37 in Schizosaccharomyces pombe tit1+ and tit1-Δ cells by using a β-galactosidase codon-swap reporter whose catalytic activity is sensitive to accurate decoding of codon 503. i6A37 increased the activity of tRNACys at a cognate codon and that of tRNATyr at a near-cognate codon, suggesting that i6A37 promotes decoding activity generally and increases fidelity at cognate codons while decreasing fidelity at noncognate codons. S. pombe cells lacking tit1+ exhibit slow growth in glycerol or rapamycin. While existing data link wobble base U34 modifications to translation of functionally related mRNAs, whether this might extend to the anticodon-adjacent position 37 was unknown. Indeed, we found a biased presence of i6A37-cognate codons in high-abundance mRNAs for ribosome subunits and energy metabolism, congruent with the observed phenotypes and the idea that i6A37 promotes translational efficiency. Polysome profiles confirmed the decreased translational efficiency of mRNAs in tit1-Δ cells. Because subsets of i6A37-tRNAs differ among species, as do their cognate codon-sensitive mRNAs, these genomic variables may underlie associated phenotypic differences.
Figures
Similar articles
-
Lack of tRNA-i6A modification causes mitochondrial-like metabolic deficiency in S. pombe by limiting activity of cytosolic tRNATyr, not mito-tRNA.RNA. 2016 Apr;22(4):583-96. doi: 10.1261/rna.054064.115. Epub 2016 Feb 8. RNA. 2016. PMID: 26857223 Free PMC article.
-
Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase: Specificity determinants and tRNA-i6A37 profiles.PLoS Genet. 2020 Apr 23;16(4):e1008330. doi: 10.1371/journal.pgen.1008330. eCollection 2020 Apr. PLoS Genet. 2020. PMID: 32324744 Free PMC article.
-
Defective i6A37 modification of mitochondrial and cytosolic tRNAs results from pathogenic mutations in TRIT1 and its substrate tRNA.PLoS Genet. 2014 Jun 5;10(6):e1004424. doi: 10.1371/journal.pgen.1004424. eCollection 2014 Jun. PLoS Genet. 2014. PMID: 24901367 Free PMC article.
-
The modified base isopentenyladenosine and its derivatives in tRNA.RNA Biol. 2017 Sep 2;14(9):1197-1208. doi: 10.1080/15476286.2017.1294309. Epub 2017 Feb 17. RNA Biol. 2017. PMID: 28277934 Free PMC article. Review.
-
The Importance of Being Modified: The Role of RNA Modifications in Translational Fidelity.Enzymes. 2017;41:1-50. doi: 10.1016/bs.enz.2017.03.005. Epub 2017 Apr 22. Enzymes. 2017. PMID: 28601219 Free PMC article. Review.
Cited by
-
A highly conserved tRNA modification contributes to C. albicans filamentation and virulence.Microbiol Spectr. 2024 May 2;12(5):e0425522. doi: 10.1128/spectrum.04255-22. Epub 2024 Apr 8. Microbiol Spectr. 2024. PMID: 38587411 Free PMC article.
-
Epigenetics and plant hormone dynamics: a functional and methodological perspective.J Exp Bot. 2024 Sep 11;75(17):5267-5294. doi: 10.1093/jxb/erae054. J Exp Bot. 2024. PMID: 38373206 Free PMC article. Review.
-
Molecular Coping Mechanisms: Reprogramming tRNAs To Regulate Codon-Biased Translation of Stress Response Proteins.Acc Chem Res. 2023 Dec 5;56(23):3504-3514. doi: 10.1021/acs.accounts.3c00572. Epub 2023 Nov 22. Acc Chem Res. 2023. PMID: 37992267
-
Posttranscriptional modification to the core of tRNAs modulates translational misreading errors.RNA. 2023 Dec 18;30(1):37-51. doi: 10.1261/rna.079797.123. RNA. 2023. PMID: 37907335
-
Research Advances on Deafness Genes Associated with Mitochondrial tRNA-37 Modifications.J Int Adv Otol. 2023 Oct;19(5):414-419. doi: 10.5152/iao.2023.231107. J Int Adv Otol. 2023. PMID: 37789629 Free PMC article. Review.
References
-
- Bjork GR. 1995. Biosynthesis and function of modified nucleosides, p 165–206 In Söll D, RajBhandary UL. (ed), tRNA: structure, biosynthesis, and function. ASM Press, Washington, DC
-
- Yokoyama S, Nishimura S. 1995. Modified nucleosides and codon recognition, p 207–223 In Söll D, RajBhandary UL. (ed), tRNA: structure, biosynthesis, and function. ASM Press, Washington, DC
-
- Agris PF, Vendeix FA, Graham WD. 2007. tRNA's wobble decoding of the genome: 40 years of modification. J. Mol. Biol. 366: 1– 13 - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources