The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

doi:10.1038/nmeth.4013

. 2016 Sep 29;13(10):805-6.

doi: 10.1038/nmeth.4013.

The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

Seth Blackshaw^{1

2

3

4

5}, Anand Venkataraman¹, Jose Irizarry⁶, Kun Yang⁷, Stephen Anderson^{8

9}, Elliot Campbell^{8

9}, Christine L Gatlin^{10

11}, Nancy L Freeman^{11

12}, Ravi Basavappa^{11

13}, Randall Stewart^{11

14}, Michael A Loss¹⁵, Ignacio Pino⁶, Heng Zhu^{4

16}, Joel S Bader^{4

17}

Affiliations

¹ Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, Maryland, USA.
² Department of Neurology, Johns Hopkins University, Baltimore, Maryland, USA.
³ Department of Ophthalmology, Johns Hopkins University, Baltimore, Maryland, USA.
⁴ Center for Human Systems Biology, Johns Hopkins University, Baltimore, Maryland, USA.
⁵ Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
⁶ CDI Laboratories, Guanajibo Research and Innovation Park, Mayaguez, Puerto Rico.
⁷ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
⁸ Department of Molecular Biology and Biochemistry, Rutgers University, New Brunswick, New Jersey, USA.
⁹ Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey, USA.
¹⁰ National Human Genome Research Institute, Bethesda, Maryland, USA.
¹¹ National Institutes of Health, Bethesda, Maryland, USA.
¹² Division of Scientific Programs, National Institute on Deafness and Other Communications Disorders, Bethesda, Maryland, USA.
¹³ Office of Strategic Coordination, Office of the Director, National Institutes of Health, Bethesda, Maryland, USA.
¹⁴ National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA.
¹⁵ Advanced Biomedical Computing Center, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, Maryland, USA.
¹⁶ Department of Pharmacology, Johns Hopkins University, Baltimore, Maryland, USA.
¹⁷ Neuroscience Center, Bethesda, Maryland, USA.

PMID: 27684578
PMCID: PMC5886368
DOI: 10.1038/nmeth.4013

The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

Seth Blackshaw et al. Nat Methods. 2016.

. 2016 Sep 29;13(10):805-6.

doi: 10.1038/nmeth.4013.

Authors

Affiliations

¹ Solomon H. Snyder Department of Neuroscience, Johns Hopkins University, Baltimore, Maryland, USA.
² Department of Neurology, Johns Hopkins University, Baltimore, Maryland, USA.
³ Department of Ophthalmology, Johns Hopkins University, Baltimore, Maryland, USA.
⁴ Center for Human Systems Biology, Johns Hopkins University, Baltimore, Maryland, USA.
⁵ Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
⁶ CDI Laboratories, Guanajibo Research and Innovation Park, Mayaguez, Puerto Rico.
⁷ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
⁸ Department of Molecular Biology and Biochemistry, Rutgers University, New Brunswick, New Jersey, USA.
⁹ Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey, USA.
¹⁰ National Human Genome Research Institute, Bethesda, Maryland, USA.
¹¹ National Institutes of Health, Bethesda, Maryland, USA.
¹² Division of Scientific Programs, National Institute on Deafness and Other Communications Disorders, Bethesda, Maryland, USA.
¹³ Office of Strategic Coordination, Office of the Director, National Institutes of Health, Bethesda, Maryland, USA.
¹⁴ National Institute of Neurological Disorders and Stroke, Bethesda, Maryland, USA.
¹⁵ Advanced Biomedical Computing Center, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, Maryland, USA.
¹⁶ Department of Pharmacology, Johns Hopkins University, Baltimore, Maryland, USA.
¹⁷ Neuroscience Center, Bethesda, Maryland, USA.

PMID: 27684578
PMCID: PMC5886368
DOI: 10.1038/nmeth.4013

No abstract available

PubMed Disclaimer

Conflict of interest statement

COMPETING FINANCIAL INTERESTS

The authors declare competing financial interests: details are available in the online version of the paper (doi:10.1038/nmeth.4013).

Figures

**Figure 1**
Flowchart showing pipeline for rAb and mAb generation and validation. All validation data are available at http://proteincapture.org.

See this image and copyright information in PMC

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[1] Bradbury A, Plückthun A. Nature. 2015;518:27–29. - PubMed

[2] Bradbury A, Plückthun A. Nature. 2015;518:27–29. - PubMed

[3] Baker M. Nature. 2015;521:274–276. - PubMed

[4] Baker M. Nature. 2015;521:274–276. - PubMed

[5] Uhlén M, et al. Science. 2015;347:1260419. - PubMed

[6] Uhlén M, et al. Science. 2015;347:1260419. - PubMed

[7] Rhodes KJ, Trimmer JS. J Neurosci. 2006;26:8017–8020. - PMC - PubMed

[8] Rhodes KJ, Trimmer JS. J Neurosci. 2006;26:8017–8020. - PMC - PubMed

[9] Jeong JS, et al. Mol Cell Proteomics. 2012;11 O111.016253. - PMC - PubMed

[10] Jeong JS, et al. Mol Cell Proteomics. 2012;11 O111.016253. - PMC - PubMed

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The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

Affiliations

The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

Authors

Affiliations

Conflict of interest statement

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