FIG. 6.
Effect of PFOA treatment on protein levels of Areg, HGF, IGF-I, ERα, EGFR, cyclin D1, and PCNA in mouse mammary glands. Wild-type and PPARα knockout mice were sacrificed 24 h after the last vehicle control or PFOA (5 mg/kg) treatment. Protein samples were prepared from frozen mammary glands and used for Western blot analysis as described in the “Materials and Methods” section. The protein levels of Areg, HGF, IGF-I, ERα, EGFR, cyclin D1, and PCNA in three individual vehicle control– or PFOA-treated mouse mammary glands were analyzed by Western blot. GAPDH served as a protein loading control. Representative Western blot from one animal in each group is shown.