FIG. 5.
Effect of PFOA treatment on protein levels of steroid hormone synthetic enzymes in mouse ovaries. Wild-type and PPARα knockout mice were sacrificed 24 h after the last vehicle control or PFOA (5 mg/kg) treatment. Protein samples prepared from frozen ovaries were used for Western blot analysis as described in the “Materials and Methods” section. The protein levels of HSD3β1, HSD17β1, and aromatase in three individual vehicle control– or PFOA-treated ovaries were analyzed by Western blot. GAPDH served as a protein loading control. Representative Western blot from one animal in each group is shown.