Narendra and Youle review the current understanding of the role of PINK1–Parkin in the quality control of mitophagy, highlighting the underlying mechanisms and physiological relevance of the pathway, as well as its role in neuroprotection.

Cell behaviour changes temporally in response to environmental and metabolic cues. This also applies to membrane contact sites (MCSs), where organelles come into close proximity to perform specific functions, such as lipid transfer or calcium signalling. Here, we discuss how MCSs change over time and whether MCSs exhibit circadian rhythmicity.

Man and Kanneganti discuss how pattern-recognition sensors in innate immune cells recognize and respond to cell-death signatures, and highlight molecular targets for potential therapeutic development.

With biomedical sciences quickly outgrowing many other application areas in terms of data generation, there is a unique opportunity for life sciences to become one of the greatest beneficiaries of research in machine learning and AI, and also inspire foundational developments in it.

Lu et al. reveal the spatiotemporal coordination between two nucleation-promoting factors, WAVE and N-WASP, and two actin-bundling proteins, dynamin and WIP, in generating invasive protrusions at the mammalian myoblast fusogenic synapse.

Zhang et al. uncover different dynamics underlying the maintenance and regulation of H3K9me3-enriched heterochromatin domains in mouse embryonic stem cells and propose that loss of H3K9me3 occurs through two distinct dynamic modes: passive dilution and active removal.

Phipps et al. report a cohesin-dependent double-strand break (DSB) DNA end-tethering mechanism that requires Exo1 and SMC5/6 in budding yeast. They show that cohesin oligomerization promotes DSB end-tethering and repair, safeguarding genomic integrity.

Matsuwaka et al. show that JARID2-PRC2 initiates H3K27me2 deposition during the eight-cell-to-morula transition, following H2AK119 mono-ubiquitylation deposition. It is also required for the subsequent H3K27me3 deposition at the blastocyst stage.

Ge, Zhou, Fu et al. find caspase-2 accumulates in biomolecular condensates with ubiquitin and proteasomal components and functions as a deubiquitinase following stress. Caspase-2-deficient mice accumulate poly-ubiquitinated TDP-43 and show motor defects.

Zhong et al. find that SERRATE (SE) regulates the m⁶A methyltransferase complex (MTC) to control m⁶A deposition, and show cross-regulation between the MTC and the SE-mediated microprocessor governs miRNA production in Arabidopsis.

Cell behaviour changes temporally in response to environmental and metabolic cues. This also applies to membrane contact sites (MCSs), where organelles come into close proximity to perform specific functions, such as lipid transfer or calcium signalling. Here, we discuss how MCSs change over time and whether MCSs exhibit circadian rhythmicity.