Supplementary Figure 1: Gating strategy for flow cytometry data. | Nature Protocols

Supplementary Figure 1: Gating strategy for flow cytometry data.

From: Heat-induced radiolabeling and fluorescence labeling of Feraheme nanoparticles for PET/SPECT imaging and flow cytometry

Supplementary Figure 1

Representative gating strategy for excluding doublets (a), and debris (b) in flow cytometry analysis of 1×10^6 events. Singlets are defined on the Forward-Scatter(height) vs. Forward-Scatter(area) dot plot (a). Singlets comprise 84.35% of total events in this data set. Small debris are excluded on a Side-Scatter(Area) vs. Forward-Scatter(Area) dot plot (b). Debris comprise 78.75% of all events in this data set. The data that was shown previously and that was used for analysis was those events that were non-debris singlets. In this data set, the non-debris singlet populations comprised 16.52% of all events.

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