Generating live attenuated vaccines through repeated passages in cells and animals has been standard procedure for more than a century. A more targeted approach may lie on the horizon (pages 154–161).
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Minor, P. A deader vaccine?. Nat Med 14, 120–121 (2008). https://doi.org/10.1038/nm0208-120
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DOI: https://doi.org/10.1038/nm0208-120