Extended Data Fig. 2: Gating strategy and representative cytokine dot plots. | Nature Immunology

Extended Data Fig. 2: Gating strategy and representative cytokine dot plots.

From: Delayed-interval BNT162b2 mRNA COVID-19 vaccination enhances humoral immunity and induces robust T cell responses

Extended Data Fig. 2

(A) The sequential gating strategy for identifying CD4+ and CD8+ T cells. Arrows indicate the sequence of hierarchical gates. Number in each plot refers to the frequency of the gated subset in the parental population. (B, C) Plots showing IFN-γ (y-axis) vs. IL-2 (x-axis) production, gated on CD4+ T cells (B) or CD8+ T cells (C). Plot are taken from the same participant. Plots show T cells stimulated with SARS-CoV-2 spike peptides, or media alone for the unstimulated control. Left two panels show response with 3-6-week interval, and right two panels show T cell responses following administration of delayed (8-16-week interval).

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