Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Somatic recombination in a murine T-cell receptor gene

Nature volume 309, pages 322–326 (1984)Cite this article

Abstract

A putative T-cell receptor gene was isolated from the DNA of the helper hybridoma, 2B4. Analysis of the sequence components of this rearranged gene indicates the presence of separate variable (V), diversity (D) and joining (J) region elements analogous to those of heavy-chain immunoglobulins. These findings further support the belief that the products of this locus are involved in antigen recognition by T cells. There is no evidence of somatic mutation between the putative germ-line and the expressed variable-region gene.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Matzinger, P. Nature 292, 497–501 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Williamson, A. R. Nature 283, 527–532 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Kappler, J. W., Skidmore, B., White, J. & Marrack, P. J. exp. Med. 153, 1198–1214 (1981).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Heber-Katz, E. et al. J. exp. Med. 155, 1086–1099 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Hunig, T. R. & Bevan, M. J. J. exp. Med. 155, 111–125 (1982).

    Article  CAS  PubMed  Google Scholar 

  6. Lonai, P. et al. J. exp. Med. 154, 1910–1921 (1981).

    Article  CAS  PubMed  Google Scholar 

  7. Elliot, B. E., Nagy, Z. A., Ben-Neriah, Y. & Givol, D. Nature 285, 496–498 (1980).

    Article  ADS  Google Scholar 

  8. Hedrick, S. M., Cohen, D. I., Nielsen, E. A. & Davis, M. M. Nature 308, 149–153 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  9. Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I. & Davis, M. M. Nature 308, 153–158 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Yanagi, Y. et al. Nature 308, 145–149 (1984).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Samelson, L. E., Germain, R. N. & Schwartz, R. H. Proc. natn. Acad. Sci. U.S.A. 80, 6972–6976 (1983).

    Article  ADS  CAS  Google Scholar 

  12. Allison, J. P., MacIntyre, B. W. & Block, D. J. Immun. 129, 2293–2300 (1982).

    CAS  PubMed  Google Scholar 

  13. Haskins, K. et al. J. exp. Med. 157, 1149–1169 (1983).

    Article  CAS  PubMed  Google Scholar 

  14. Benoist, C. O., Mathis, D. J., Kanter, M. R., Williams, V. F. & McDevitt, H. O. Proc. natn. Acad. Sci. U.S.A. 80, 534–538 (1983).

    Article  ADS  CAS  Google Scholar 

  15. Huynh, T. V., Young, R. A. & Davis, R. W. in DNA Cloning Techniques: A Practical Approach (ed. Glover, D. M.) (IRL Press, Oxford, 1984).

    Google Scholar 

  16. Kabat, E. A., Wu, T. T., Bilofsky, H., Reid-Miller, M. & Perry, H. in Sequences of Proteins of Immunological Interest (US Dept of Health and Human Services, Washington, DC, 1983).

    Google Scholar 

  17. Maniatis, T. et al. Cell 15, 687–701 (1978).

    Article  CAS  PubMed  Google Scholar 

  18. Viera, J. & Messing, J. Gene 19, 259–268 (1982).

    Article  Google Scholar 

  19. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  PubMed  Google Scholar 

  20. Parslow, T. G., Blair, D. L., Murphy, W. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. (in the press).

  21. Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).

    Article  CAS  PubMed  Google Scholar 

  22. Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–682 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Schilling, J., Clevinger, B., Davie, J. M. & Hood, L. Nature 283, 35–40 (1980).

    Article  ADS  CAS  PubMed  Google Scholar 

  24. Kurosawa, Y. & Tonegawa, S. J. exp. Med. 155, 201–218 (1982).

    Article  CAS  PubMed  Google Scholar 

  25. Sakano, H., Kurosawa, Y., Weigart, M. & Tonegawa, S. Nature 290, 562–579 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  26. Alt, F. W. & Baltimore, D. Proc. natn. Acad. Sci. U.S.A. 79, 4118–4122 (1982).

    Article  ADS  CAS  Google Scholar 

  27. Hood, L. et al. Cold Spring Harb. Symp. quant. Biol. 45, 887–898 (1981).

    Article  CAS  PubMed  Google Scholar 

  28. Jerne, N. K. Eur. J. Immun. 1, 1–9 (1971).

    Article  CAS  Google Scholar 

  29. Kaye, J. & Janeway, C. J. exp. Med. (submitted).

  30. Sakano, H., Huppi, G., Heinrich, G. & Tonegawa, S. Nature 280, 288–294 (1979).

    Article  ADS  CAS  PubMed  Google Scholar 

  31. Max, E. E., Seidman, J. G. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 76, 3450–3454 (1979).

    Article  ADS  CAS  Google Scholar 

  32. Amzel, M., Poljak, R. J., Saul, F., Varga, J. M. & Richards, F. F. Proc. natn. Acad. Sci. U.S.A. 71, 1427–1430 (1974).

    Article  ADS  CAS  Google Scholar 

  33. Davies, D. R., Padlan, E. A. & Segal, D. A. Rev. Biochem. 44, 629–667 (1975).

    Article  Google Scholar 

  34. Acuto, O. et al. Cell 34, 717–726 (1983).

    Article  CAS  PubMed  Google Scholar 

  35. McIntyre, B. W. & Allison, J. P. Cell 34, 739–745 (1983).

    Article  CAS  PubMed  Google Scholar 

  36. Kappler, J., Kubo, R., Haskins, K., White, J. & Marrack, P. Cell 34, 727–737 (1983).

    Article  CAS  PubMed  Google Scholar 

  37. Marrack, P. et al. J. exp. Med. 158, 1077–1091 (1983).

    Article  CAS  PubMed  Google Scholar 

  38. Swain, S. L. Immun. Rev. 74, 129–142 (1983).

    Article  CAS  PubMed  Google Scholar 

  39. Mullins, J. I., Brody, D. S., Binari, R. C. & Cotter, S. M. Nature (in the press).

  40. Clayberger, C., Dekruyff, R. H., Aisenberg, J. & Cantor, H. J. exp. Med. 157, 1906–1919 (1983).

    Article  CAS  PubMed  Google Scholar 

  41. Fresno, M., Nabel, G., McVay-Bordreau, L., Furthmayer, H. & Cantor, H. J. exp. Med. 153, 1246–1259 (1981).

    Article  CAS  PubMed  Google Scholar 

  42. Gascoigne, N. R. J., Chien, Y.-h., Becker, D., Kavaler, J. & Davis, M. M. Nature (submitted).

  43. Lee, N. E. et al. J. exp. Med. (submitted).

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, School of Medicine, Stanford University, Stanford, California, 94305, USA

    Yueh-hsiu Chien, Nicholas R. J. Gascoigne, Joshua Kavaler, Nadine E. Lee & Mark M. Davis

Authors
  1. Yueh-hsiu Chien
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nicholas R. J. Gascoigne
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joshua Kavaler
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Nadine E. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mark M. Davis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chien, Yh., Gascoigne, N., Kavaler, J. et al. Somatic recombination in a murine T-cell receptor gene. Nature 309, 322–326 (1984). https://doi.org/10.1038/309322a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/309322a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing