(genome-assembly) [mchege@crcfe01 fastq]$ ~/.conda/envs/genome-assembly/bin/megahit -1 ERR2231568_1.fastqsanger.gz -2 ERR2231568_2.fastqsanger.gz -t 8 --k-min 21 --k-max 91 --k-step 12 -o megahit_result
2023-05-15 06:17:08 - MEGAHIT v1.2.9
2023-05-15 06:17:08 - Using megahit_core with POPCNT and BMI2 support
Traceback (most recent call last):
File "/afs/crc.nd.edu/user/m/mchege/.conda/envs/genome-assembly/bin/megahit", line 1038, in
main()
File "/afs/crc.nd.edu/user/m/mchege/.conda/envs/genome-assembly/bin/megahit", line 988, in main
build_library()
File "/afs/crc.nd.edu/user/m/mchege/.conda/envs/genome-assembly/bin/megahit", line 264, in checked_or_call
func(*args, **kwargs)
File "/afs/crc.nd.edu/user/m/mchege/.conda/envs/genome-assembly/bin/megahit", line 723, in build_library
create_fifo('pe1', i, inpipe_cmd(opt.pe1[i]))
File "/afs/crc.nd.edu/user/m/mchege/.conda/envs/genome-assembly/bin/megahit", line 710, in create_fifo
os.mkfifo(fifo_path)
PermissionError: [Errno 1] Operation not permitted