The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human  THP-1 Macrophages

Robertson, Ruairi C.; Guihéneuf, Freddy; Bahar, Bojlul; Schmid, Matthias; Stengel, Dagmar B.; Fitzgerald, Gerald F.; Ross, R. Paul; Stanton, Catherine

doi:10.3390/md13085402

Cited by 144 publications

(115 citation statements)

References 71 publications

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“…Glycolipids have already been identified for members of the Rhodophyta (red seaweeds), namely in the genus Gracilaria [21,30,40,41,42]. However, the majority of published works only identified a few species of glycolipids, either by using offline TLC–MS [30,40,42,43] or selected solvent extraction and MS analysis [15,20,29]. More recently, a detailed profile of Chondrus crispus was reported using LC–MS and MS/MS [26].…”

Section: Resultsmentioning

confidence: 99%

“…Polar lipids are nowadays recognized as an important reservoir of fatty acids with nutritional value, e.g., n -3 FAs [5,6], and they are also considered high-value novel lipids with beneficial health effects such as antitumoral [7,8], antiviral [8,9], antifungal [10], antibacterial [11], and anti-inflammatory [11,12], with potential applications in the nutraceutical and pharmaceutical industries [7,8,11]. However, in spite of their recognized potential, they are still scarcely studied [12,13,14,15]. Some studies reported that polar lipids isolated from seaweeds can promote growth-inhibiting effects on human hepatocellular carcinoma cell lines (HepG2) [16] and thus can act as inhibitors of DNA polymerases with capability to inhibit tumor cell proliferation [17].…”

Section: Introductionmentioning

confidence: 99%

“…Some studies reported that polar lipids isolated from seaweeds can promote growth-inhibiting effects on human hepatocellular carcinoma cell lines (HepG2) [16] and thus can act as inhibitors of DNA polymerases with capability to inhibit tumor cell proliferation [17]. Moreover, they have been associated with anti-inflammatory properties through the inhibition of pro-inflammatory cytokines interleukin IL-6 and IL-8 production [15] and/or by the inhibition of nitric oxide (NO) production [18,19,20,21]. Lipid-based agents are therefore emerging molecules in therapeutics aimed to regulate inflammatory pathways or even impair downstream tumorigenic processes [22,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

et al. 2017

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The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC–MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

et al. 2017

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“…Full details of the arrays were previously described [22]. Gene expression profiling for human cytokines/chemokines was performed by using an in-house PCR array that consisted of 89 genes [23].…”

Section: Methodsmentioning

confidence: 99%

Gene Expression Profiling and Functional Characterization of Macrophages in Response to Circulatory Microparticles Produced during <b><i>Trypanosoma cruzi</i></b> Infection and Chagas Disease

Chowdhury

Koo²,

Gupta

et al. 2016

J Innate Immun

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Background: Chronic inflammation and oxidative stress are hallmarks of chagasic cardiomyopathy (CCM). In this study, we determined if microparticles (MPs) generated during Trypanosoma cruzi (Tc) infection carry the host's signature of the inflammatory/oxidative state and provide information regarding the progression of clinical disease. Methods: MPs were harvested from supernatants of human peripheral blood mononuclear cells in vitro incubated with Tc (control: LPS treated), plasma of seropositive humans with a clinically asymptomatic (CA) or symptomatic (CS) disease state (vs. normal/healthy [NH] controls), and plasma of mice immunized with a protective vaccine before challenge infection (control: unvaccinated/infected). Macrophages (mφs) were incubated with MPs, and we probed the gene expression profile using the inflammatory signaling cascade and cytokine/chemokine arrays, phenotypic markers of mφ activation by flow cytometry, cytokine profile by means of an ELISA and Bioplex assay, and oxidative/nitrosative stress and mitotoxicity by means of colorimetric and fluorometric assays. Results:Tc- and LPS-induced MPs stimulated proliferation, inflammatory gene expression profile, and nitric oxide (∙NO) release in human THP-1 mφs. LPS-MPs were more immunostimulatory than Tc-MPs. Endothelial cells, T lymphocytes, and mφs were the major source of MPs shed in the plasma of chagasic humans and experimentally infected mice. The CS and CA (vs. NH) MPs elicited >2-fold increase in NO and mitochondrial oxidative stress in THP-1 mφs; however, CS (vs. CA) MPs elicited a more pronounced and disease-state-specific inflammatory gene expression profile (IKBKB, NR3C1, and TIRAP vs. CCR4, EGR2, and CCL3), cytokine release (IL-2 + IFN-γ > GCSF), and surface markers of mφ activation (CD14 and CD16). The circulatory MPs of nonvaccinated/infected mice induced 7.5-fold and 40% increases in ∙NO and IFN-γ production, respectively, while these responses were abolished when RAW264.7 mφs were incubated with circulatory MPs of vaccinated/infected mice. Conclusion: Circulating MPs reflect in vivo levels of an oxidative, nitrosative, and inflammatory state, and have potential utility in evaluating disease severity and the efficacy of vaccines and drug therapies against CCM.

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“…Natural products are alternatives to these drugs and offer hope for discovering bioactive lead compounds that may be developed into drugs for treatment of inflammatory disorders [12]. Plenty of unique marine natural products and their derivatives, such as sesquiterpenoid, polysaccharide, steroid/sterol and alkaloid, are found to manifest an anti-inflammatory action [13,14,15,16,17,18]. …”

Section: Introductionmentioning

confidence: 99%

Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression

et al. 2016

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In order to find new natural products with anti-inflammatory activity, chemical investigation of a 3000-meter deep-sea sediment derived bacteria Bacillus subtilis B5 was carried out. A new macrolactin derivative was isolated and identified as 7,13-epoxyl-macrolactin A (1). Owing to the existence of the epoxy ring, 1 exhibited a significant inhibitory effect on the expression of inducible nitric oxide and cytokines, compared with previously isolated known macrolactins (2–5). Real-time Polymerase Chain Reaction (PCR) analysis showed that the new compound significantly inhibited the mRNA expressions of inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Reverse transcription-PCR analysis demonstrated that the new compound reduced the mRNA expression level of IL-1β in a concentration-dependent manner.

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The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages

Cited by 144 publications

References 71 publications

Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity

Gene Expression Profiling and Functional Characterization of Macrophages in Response to Circulatory Microparticles Produced during <b><i>Trypanosoma cruzi</i></b> Infection and Chagas Disease

Macrolactins from Marine-Derived Bacillus subtilis B5 Bacteria as Inhibitors of Inducible Nitric Oxide and Cytokines Expression

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