Regulation of tyrosine hydroxylase mRNA stability by protein-binding, pyrimidine-rich sequence in the 3'-untranslated region
- PMID: 9891025
- DOI: 10.1074/jbc.274.4.2532
Regulation of tyrosine hydroxylase mRNA stability by protein-binding, pyrimidine-rich sequence in the 3'-untranslated region
Abstract
The stability of mRNA for tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine synthesis, is regulated by oxygen tension in the pheochromocytoma-derived PC12 cell line. We previously identified a pyrimidine-rich 27-base-long protein-binding sequence in the 3'-untranslated region of TH mRNA that is associated with hypoxia-inducible formation of a ribonucleoprotein complex (hypoxia-inducible protein-binding site (HIPBS)). In this study, we show that HIPBS is an mRNA stabilizing element necessary for both constitutive and hypoxia-regulated stability of TH mRNA. The mutations within this sequence that abolish protein binding markedly decrease constitutive TH mRNA stability and ablate its hypoxic regulation. A short fragment of TH mRNA that contains the wild-type HIPBS confers the increased mRNA stability to the reporter chloramphenicol acetyltransferase mRNA. However, it is not sufficient to confer hypoxic regulation. The HIPBS element binds two isoforms of a 40-kDa poly(C)-binding protein (PCBP). Hypoxia induces expression of the isoform 1, PCBP1, but not the isoform 2, PCBP2, in PC12 cells.
Similar articles
-
Characterization of the hypoxia-inducible protein binding site within the pyrimidine-rich tract in the 3'-untranslated region of the tyrosine hydroxylase mRNA.J Biol Chem. 1996 Feb 9;271(6):3293-9. doi: 10.1074/jbc.271.6.3293. J Biol Chem. 1996. PMID: 8621733
-
Post-transcriptional regulation of tyrosine hydroxylase gene expression by oxygen in PC12 cells.Kidney Int. 1997 Feb;51(2):585-90. doi: 10.1038/ki.1997.84. Kidney Int. 1997. PMID: 9027744
-
Hypoxia stimulates binding of a cytoplasmic protein to a pyrimidine-rich sequence in the 3'-untranslated region of rat tyrosine hydroxylase mRNA.J Biol Chem. 1994 Apr 1;269(13):9940-5. J Biol Chem. 1994. PMID: 7908289
-
Structural and functional analysis of hypoxia-inducible factor 1.Kidney Int. 1997 Feb;51(2):553-5. doi: 10.1038/ki.1997.77. Kidney Int. 1997. PMID: 9027737 Review.
-
Regulation of tyrosine hydroxylase mRNA stability by oxygen in PC12 cells.Adv Exp Med Biol. 1996;410:143-50. doi: 10.1007/978-1-4615-5891-0_21. Adv Exp Med Biol. 1996. PMID: 9030291 Review. No abstract available.
Cited by
-
Regulated alpha-globin mRNA decay is a cytoplasmic event proceeding through 3'-to-5' exosome-dependent decapping.RNA. 2002 Dec;8(12):1526-37. RNA. 2002. PMID: 12515385 Free PMC article.
-
Complex molecular regulation of tyrosine hydroxylase.J Neural Transm (Vienna). 2014 Dec;121(12):1451-81. doi: 10.1007/s00702-014-1238-7. Epub 2014 May 28. J Neural Transm (Vienna). 2014. PMID: 24866693 Review.
-
Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs.Cell Mol Life Sci. 2015 Sep;72(18):3575-86. doi: 10.1007/s00018-015-1905-3. Epub 2015 Apr 17. Cell Mol Life Sci. 2015. PMID: 25894693 Free PMC article.
-
Serine-threonine kinase receptor-associated protein (STRAP) regulates translation of type I collagen mRNAs.Mol Cell Biol. 2013 Oct;33(19):3893-906. doi: 10.1128/MCB.00195-13. Epub 2013 Aug 5. Mol Cell Biol. 2013. PMID: 23918805 Free PMC article.
-
Crystal structure of the third KH domain of human poly(C)-binding protein-2 in complex with a C-rich strand of human telomeric DNA at 1.6 A resolution.Nucleic Acids Res. 2007;35(8):2651-60. doi: 10.1093/nar/gkm139. Epub 2007 Apr 10. Nucleic Acids Res. 2007. PMID: 17426136 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
