Two domains of rat galectin-4 bind to distinct structures of the intercellular borders of colorectal epithelia
- PMID: 9857214
- DOI: 10.1177/002215549904700108
Two domains of rat galectin-4 bind to distinct structures of the intercellular borders of colorectal epithelia
Abstract
Galectin-4 (G4) is a member of a family of soluble galactoside-binding lectins found in various mammalian tissues. To determine the function of this protein in colorectal tissue, we separately produced the N- and C-terminal carbohydrate binding domains (CBD) of rat G4 as a recombinant glutathione S-transferase (GST) fusion protein (G4-N and G4-C) and examined the tissue binding site(s) of each CBD by light and electron microscopy (LM and EM). At the LM level, both fusion proteins stained the intercellular borders of the surface-lining epithelial cells of colorectal mucosa. At the EM level, two proteins recognized spatially close but distinct subcellular structures. G4-N stained electron-lucent flocculent substances freely located in the intercellular spaces, whereas G4-C bound to the lateral cell membranes demarcating the intercellular spaces. These findings suggest that colorectal G4 may be involved in crosslinking the lateral cell membranes of the surface-lining epithelial cells, thereby reinforcing epithelial integrity against mechanical stress exerted by the bowel lumen. (J Histochem Cytochem 47:75-82, 1999)
Similar articles
-
Recombinant galectin-1 recognizes mucin and epithelial cell surface glycocalyces of gastrointestinal tract.J Histochem Cytochem. 1997 Feb;45(2):275-83. doi: 10.1177/002215549704500212. J Histochem Cytochem. 1997. PMID: 9016316
-
Recombinant expression of a galectin from the sheep gastrointestinal parasite Teladorsagia circumcincta: its use in isolating galectin-glycoconjugates.Protein Expr Purif. 1999 Jun;16(1):152-9. doi: 10.1006/prep.1999.1057. Protein Expr Purif. 1999. PMID: 10336873
-
Soluble lactose-binding lectin from rat intestine with two different carbohydrate-binding domains in the same peptide chain.J Biol Chem. 1993 Mar 15;268(8):5929-39. J Biol Chem. 1993. PMID: 8449956
-
Distribution of anionic sites during increasing tight junctional permeability in the rat submandibular gland.J Osaka Dent Univ. 1993 Apr;27(1):23-35. J Osaka Dent Univ. 1993. PMID: 7505320
-
Isotonic water transport in secretory epithelia.Yale J Biol Med. 1977 Mar-Apr;50(2):153-63. Yale J Biol Med. 1977. PMID: 331693 Free PMC article. Review.
Cited by
-
Probing sulfatide-tissue lectin recognition with functionalized glycodendrimersomes.iScience. 2020 Dec 10;24(1):101919. doi: 10.1016/j.isci.2020.101919. eCollection 2021 Jan 22. iScience. 2020. PMID: 33409472 Free PMC article.
-
Galectin-4 Interaction with CD14 Triggers the Differentiation of Monocytes into Macrophage-like Cells via the MAPK Signaling Pathway.Immune Netw. 2019 May 29;19(3):e17. doi: 10.4110/in.2019.19.e17. eCollection 2019 Jun. Immune Netw. 2019. PMID: 31281714 Free PMC article.
-
Immunohistochemical and in situ hybridization analysis of galectin-3, a beta-galactoside binding lectin, in the urinary system of adult mice.Histochem Cell Biol. 2006 Jul;126(1):45-56. doi: 10.1007/s00418-005-0142-5. Epub 2006 Jan 11. Histochem Cell Biol. 2006. PMID: 16404573
-
Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.J Cell Biol. 2005 May 9;169(3):491-501. doi: 10.1083/jcb.200407073. J Cell Biol. 2005. PMID: 15883199 Free PMC article.
-
Galectin-4 in normal tissues and cancer.Glycoconj J. 2004;20(4):247-55. doi: 10.1023/B:GLYC.0000025819.54723.a0. Glycoconj J. 2004. PMID: 15115909 Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials