yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex
- PMID: 9844640
- DOI: 10.1016/s1097-2765(00)80166-5
yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex
Abstract
We obtained a recessive insertion mutation in the gene encoding yeast TBP-associated factor yTAFII61/68 that impairs Gcn4p-independent and Gcn4p-activated HIS3 transcription. This mutation also reduces transcription of seven other class II genes, thus indicating a broad role for this yTAFII in RNA polymerase II transcription. The Gcn4p activation domain interacts with multiple components of the SAGA complex in cell extracts, including the yTAFII proteins associated with SAGA, but not with two yTAFIIs restricted to TFIID. The taf61-1 mutation impairs binding of Gcn4p to SAGA/yTAFII subunits but not to components of holoenzyme mediator. Our results provide strong evidence that recruitment of SAGA, in addition to holoenzyme, is crucial for activation by Gcn4p in vivo and that yTAFII61 plays a key role in this process.
Similar articles
-
The Gcn4p activation domain interacts specifically in vitro with RNA polymerase II holoenzyme, TFIID, and the Adap-Gcn5p coactivator complex.Mol Cell Biol. 1998 Mar;18(3):1711-24. doi: 10.1128/MCB.18.3.1711. Mol Cell Biol. 1998. PMID: 9488488 Free PMC article.
-
Simultaneous recruitment of coactivators by Gcn4p stimulates multiple steps of transcription in vivo.Mol Cell Biol. 2005 Jul;25(13):5626-38. doi: 10.1128/MCB.25.13.5626-5638.2005. Mol Cell Biol. 2005. PMID: 15964818 Free PMC article.
-
Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA.Mol Cell. 1998 Nov;2(5):653-61. doi: 10.1016/s1097-2765(00)80163-x. Mol Cell. 1998. PMID: 9844637
-
Activation and repression mechanisms in yeast.Cold Spring Harb Symp Quant Biol. 1998;63:413-21. doi: 10.1101/sqb.1998.63.413. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384306 Review. No abstract available.
-
Mechanisms of transcriptional activation and repression can both involve TFIID.Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):517-26. doi: 10.1098/rstb.1996.0050. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735274 Review.
Cited by
-
The eukaryotic initiation factor (eIF) 5 HEAT domain mediates multifactor assembly and scanning with distinct interfaces to eIF1, eIF2, eIF3, and eIF4G.Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16164-9. doi: 10.1073/pnas.0507960102. Epub 2005 Oct 27. Proc Natl Acad Sci U S A. 2005. PMID: 16254050 Free PMC article.
-
UPS writes a new saga of SAGA.Biochim Biophys Acta Gene Regul Mech. 2023 Dec;1866(4):194981. doi: 10.1016/j.bbagrm.2023.194981. Epub 2023 Aug 30. Biochim Biophys Acta Gene Regul Mech. 2023. PMID: 37657588 Free PMC article. Review.
-
A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo.Mol Cell Biol. 2003 Apr;23(8):2800-20. doi: 10.1128/MCB.23.8.2800-2820.2003. Mol Cell Biol. 2003. PMID: 12665580 Free PMC article.
-
Components of the SAGA histone acetyltransferase complex are required for repressed transcription of ARG1 in rich medium.Mol Cell Biol. 2002 Jun;22(12):4033-42. doi: 10.1128/MCB.22.12.4033-4042.2002. Mol Cell Biol. 2002. PMID: 12024017 Free PMC article.
-
Targets of the Gal4 transcription activator in functional transcription complexes.Mol Cell Biol. 2005 Oct;25(20):9092-102. doi: 10.1128/MCB.25.20.9092-9102.2005. Mol Cell Biol. 2005. PMID: 16199885 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
