Large dense-core vesicle exocytosis in PC12 cells
- PMID: 9790867
- DOI: 10.1006/meth.1998.0668
Large dense-core vesicle exocytosis in PC12 cells
Abstract
A current major challenge in the study of regulated exocytosis is the identification of essential proteins that mediate the transit of secretory vesicles through trafficking stages such as recruitment, docking, and fusion. Defining the physiological roles and mechanisms of action of these essential proteins is paramount. The reconstitution of stages of regulated exocytosis in cell-free systems provides the opportunity to identify required proteins and establish their stage-specific mechanisms of action. PC12 cells, clonal cell lines of adrenal medullary origin, possess large dense-core vesicles that retain their competence for regulated exocytosis in a variety of permeable cell and isolated membrane preparations. We describe several cell-free systems for studies of regulated exocytosis derived from PC12 cells.
Copyright 1998 Academic Press.
Similar articles
-
Synaptotagmin function in dense core vesicle exocytosis studied in cracked PC12 cells.Nat Neurosci. 2002 Jul;5(7):649-56. doi: 10.1038/nn869. Nat Neurosci. 2002. PMID: 12055633
-
Differential properties of GTP- and Ca(2+)-stimulated exocytosis from large dense core vesicles.Traffic. 2006 Apr;7(4):416-28. doi: 10.1111/j.1600-0854.2006.00394.x. Traffic. 2006. PMID: 16536740
-
Synaptotagmin VII modulates the kinetics of dense-core vesicle exocytosis in PC12 cells.Genes Cells. 2007 Apr;12(4):511-9. doi: 10.1111/j.1365-2443.2007.01070.x. Genes Cells. 2007. PMID: 17397398
-
Mechanisms of exocytosis.Acta Physiol (Oxf). 2008 Feb;192(2):185-93. doi: 10.1111/j.1748-1716.2007.01803.x. Epub 2007 Nov 15. Acta Physiol (Oxf). 2008. PMID: 18005396 Review.
-
Secretory granule exocytosis.Physiol Rev. 2003 Apr;83(2):581-632. doi: 10.1152/physrev.00031.2002. Physiol Rev. 2003. PMID: 12663867 Review.
Cited by
-
The tandem C2 domains of synaptotagmin contain redundant Ca2+ binding sites that cooperate to engage t-SNAREs and trigger exocytosis.J Cell Biol. 2001 Sep 17;154(6):1117-23. doi: 10.1083/jcb.200105020. Epub 2001 Sep 10. J Cell Biol. 2001. PMID: 11551981 Free PMC article.
-
Identification of synaptotagmin effectors via acute inhibition of secretion from cracked PC12 cells.J Cell Biol. 2003 Jul 21;162(2):199-209. doi: 10.1083/jcb.200302060. Epub 2003 Jul 14. J Cell Biol. 2003. PMID: 12860971 Free PMC article.
-
Unexpected Ca2+-binding properties of synaptotagmin 9.Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2554-9. doi: 10.1073/pnas.0308477100. Proc Natl Acad Sci U S A. 2004. PMID: 14983047 Free PMC article.
-
Synaptotagmin-Ca2+ triggers two sequential steps in regulated exocytosis in rat PC12 cells: fusion pore opening and fusion pore dilation.J Physiol. 2006 Jan 15;570(Pt 2):295-307. doi: 10.1113/jphysiol.2005.097378. Epub 2005 Nov 17. J Physiol. 2006. PMID: 16293646 Free PMC article.
-
CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation.J Biol Chem. 2009 Jul 10;284(28):18707-14. doi: 10.1074/jbc.M109.017483. Epub 2009 May 21. J Biol Chem. 2009. PMID: 19460754 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
