Microtubule-damaging drugs triggered bcl2 phosphorylation-requirement of phosphorylation on both serine-70 and serine-87 residues of bcl2 protein
- PMID: 9735392
- DOI: 10.3892/ijo.13.4.659
Microtubule-damaging drugs triggered bcl2 phosphorylation-requirement of phosphorylation on both serine-70 and serine-87 residues of bcl2 protein
Abstract
Specifically anti-microtubule agents such as taxol, vincristine, vinblastine and dolastatin can trigger Bcl2 phosphorylation at G2-M phase of the cell cycle in malignant cells derived from a variety of human cancers. In this study, the status of Bcl2 phosphorylation was investigated in response to more antimicrotubule agents such as colchicine, colcemid or podophyllotoxin. Although these agents are not currently used for cancer therapy, they were able to trigger Bcl2 phosphorylation with simultaneous apoptosis in cancer cells. Previously, by using extensive site-directed mutagenesis studies we determined that mutation of serine-70 to alanine could not completely abrogate taxol induced Bcl2 phosphorylation. Studies reported here clearly indicate that serine-87 residue along with serine-70 of Bcl2 protein are necessary for microtubule damaging drug induced phosphorylation.
Similar articles
-
Bcl-xL is phosphorylated in malignant cells following microtubule disruption.Cancer Res. 1998 Aug 1;58(15):3331-8. Cancer Res. 1998. PMID: 9699663
-
Serine-70 is one of the critical sites for drug-induced Bcl2 phosphorylation in cancer cells.Cancer Res. 1998 Apr 15;58(8):1609-15. Cancer Res. 1998. PMID: 9563469
-
Raf-1/bcl-2 phosphorylation: a step from microtubule damage to cell death.Cancer Res. 1997 Jan 1;57(1):130-5. Cancer Res. 1997. PMID: 8988053
-
The effect of antimicrotubule agents on signal transduction pathways of apoptosis: a review.Cancer Chemother Pharmacol. 1999;44(5):355-61. doi: 10.1007/s002800050989. Cancer Chemother Pharmacol. 1999. PMID: 10501907 Review.
-
Regulation of Bcl2 phosphorylation and potential significance for leukemic cell chemoresistance.J Natl Cancer Inst Monogr. 2001;(28):30-7. doi: 10.1093/oxfordjournals.jncimonographs.a024254. J Natl Cancer Inst Monogr. 2001. PMID: 11158204 Review.
Cited by
-
ID1 enhances docetaxel cytotoxicity in prostate cancer cells through inhibition of p21.Cancer Res. 2010 Apr 15;70(8):3239-48. doi: 10.1158/0008-5472.CAN-09-3186. Epub 2010 Apr 13. Cancer Res. 2010. PMID: 20388787 Free PMC article.
-
Roles of the Raf/MEK/ERK pathway in cell growth, malignant transformation and drug resistance.Biochim Biophys Acta. 2007 Aug;1773(8):1263-84. doi: 10.1016/j.bbamcr.2006.10.001. Epub 2006 Oct 7. Biochim Biophys Acta. 2007. PMID: 17126425 Free PMC article. Review.
-
Proteasomal degradation of human peptidyl prolyl isomerase pin1-pointing phospho Bcl2 toward dephosphorylation.Neoplasia. 2002 May-Jun;4(3):218-27. doi: 10.1038/sj.neo.7900233. Neoplasia. 2002. PMID: 11988841 Free PMC article.
-
Aspirin-induced Bcl-2 translocation and its phosphorylation in the nucleus trigger apoptosis in breast cancer cells.Exp Mol Med. 2013 Oct 11;45(10):e47. doi: 10.1038/emm.2013.91. Exp Mol Med. 2013. PMID: 24113271 Free PMC article.
-
Microtubule-targeting drugs induce bcl-2 phosphorylation and association with Pin1.Neoplasia. 2001 Nov-Dec;3(6):550-9. doi: 10.1038/sj.neo.7900213. Neoplasia. 2001. PMID: 11774038 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
