Crystallographic Characterization of the DNA-Binding Domain of Interferon Regulatory Factor-2 Complexed with DNA
- PMID: 9705880
- DOI: 10.1006/jsbi.1998.3970
Crystallographic Characterization of the DNA-Binding Domain of Interferon Regulatory Factor-2 Complexed with DNA
Abstract
Interferon regulatory factors (IRFs) are transcription factors for interferon-related genes, which manifest both antiviral and tumor-suppressor activities and regulate cell growth in response to DNA damage. For the transcription initiation of the interferon-beta gene, IRFs form a macromolecular assembly bound to the promoter DNA, referred to as an enhancesome, together with several other transcription factors and DNA-binding proteins. The three-dimensional structure of IRF-DNA complex would provide insights into the structure and function of the enhancesome. In this study, we crystallized the DNA-binding domain of interferon regulatory factor-2 complexed with a DNA fragment. The crystals reproducibly grew by the vapor diffusion technique with 2-methyl-pentanediol from solutions containing small detergents, such as n-octyl-beta-d-glucoside. Cryocrystallographic experiments showed that crystals belong to space group P212121 with a = 90.66 Å, b = 101.01 Å, c = 171.58 Å and diffract up to 2.8 Å resolution. The absorption measurements of a solution in which the crystals were dissolved indicate that the DNA-binding domain binds to the DNA as a dimer. The calculated values of the solvent contents suggest that the protein-DNA complexes form a multimer in the crystal. These features may reflect the association of the complexes in the enhancesome. Copyright 1998 Academic Press.
Similar articles
-
Molecular characterization of IRF3 and IRF7 in rainbow trout, Oncorhynchus mykiss: functional analysis and transcriptional modulation.Mol Immunol. 2008 Dec;46(2):269-85. doi: 10.1016/j.molimm.2008.08.265. Epub 2008 Sep 20. Mol Immunol. 2008. PMID: 18805586
-
Interferon-gamma regulation of the human mimecan promoter.Mol Vis. 2003 Jun 30;9:277-87. Mol Vis. 2003. PMID: 12835654
-
The NMR solution structure of a mutant of the Max b/HLH/LZ free of DNA: insights into the specific and reversible DNA binding mechanism of dimeric transcription factors.J Mol Biol. 2004 Sep 17;342(3):813-32. doi: 10.1016/j.jmb.2004.07.058. J Mol Biol. 2004. PMID: 15342239
-
Structure of IRF-3 bound to the PRDIII-I regulatory element of the human interferon-beta enhancer.Mol Cell. 2007 Jun 8;26(5):703-16. doi: 10.1016/j.molcel.2007.04.022. Mol Cell. 2007. PMID: 17560375
-
Binding of Kaposi's sarcoma-associated herpesvirus K-bZIP to interferon-responsive factor 3 elements modulates antiviral gene expression.J Virol. 2007 Oct;81(20):10950-60. doi: 10.1128/JVI.00183-07. Epub 2007 Jul 25. J Virol. 2007. PMID: 17652396 Free PMC article.
Cited by
-
Expression and DNA binding activity of the recombinant interferon regulatory factor-1 (IRF-1) of mouse.Mol Biol Rep. 2005 Jun;32(2):103-16. doi: 10.1007/s11033-004-6940-3. Mol Biol Rep. 2005. PMID: 16022283
-
STAT1 and pathogens, not a friendly relationship.Biochimie. 2010 May;92(5):425-44. doi: 10.1016/j.biochi.2010.02.009. Epub 2010 Feb 13. Biochimie. 2010. PMID: 20159032 Free PMC article. Review.
-
IRF7: activation, regulation, modification and function.Genes Immun. 2011 Sep;12(6):399-414. doi: 10.1038/gene.2011.21. Epub 2011 Apr 14. Genes Immun. 2011. PMID: 21490621 Free PMC article. Review.
-
Interferon regulatory factors: critical mediators of human lupus.Transl Res. 2015 Feb;165(2):283-95. doi: 10.1016/j.trsl.2014.10.002. Epub 2014 Oct 13. Transl Res. 2015. PMID: 25445206 Free PMC article. Review.
LinkOut - more resources
Full Text Sources
