Functional analysis of the mouse Scn8a sodium channel
- PMID: 9698304
- PMCID: PMC6793187
- DOI: 10.1523/JNEUROSCI.18-16-06093.1998
Functional analysis of the mouse Scn8a sodium channel
Abstract
The mouse Scn8a sodium channel and its ortholog Na6 in the rat are abundantly expressed in the CNS. Mutations in mouse Scn8a result in neurological disorders, including paralysis, ataxia, and dystonia. In addition, Scn8a has been observed to mediate unique persistent and resurgent currents in cerebellar Purkinje cells (Raman et al., 1997). To examine the functional characteristics of this channel, we constructed a full-length cDNA clone encoding the mouse Scn8a sodium channel and expressed it in Xenopus oocytes. The electrophysiological properties of the Scn8a channels were compared with those of the Rat1 and Rat2 sodium channels. Scn8a channels were sensitive to tetrodotoxin at a level comparable to that of Rat1 or Rat2. Scn8a channels inactivated more rapidly and showed differences in their voltage-dependent properties compared with Rat1 and Rat2 when only the alpha subunits were expressed. Coexpression of the beta1 and beta2 subunits modulated the properties of Scn8a channels, but to a lesser extent than for the Rat1 or Rat2 channels. Therefore, all three channels showed similar voltage dependence and inactivation kinetics in the presence of the beta subunits. Scn8a channels coexpressed with the beta subunits exhibited a persistent current that became larger with increasing depolarization, which was not observed for either Rat1 or Rat2 channels. The unique persistent current observed for Scn8a channels is consistent with the hypothesis that this channel is responsible for distinct sodium conductances underlying repetitive firing of action potentials in Purkinje neurons.
Figures







Similar articles
-
Functional analysis of the rat I sodium channel in xenopus oocytes.J Neurosci. 1998 Feb 1;18(3):811-20. doi: 10.1523/JNEUROSCI.18-03-00811.1998. J Neurosci. 1998. PMID: 9437003 Free PMC article.
-
The absence of resurgent sodium current in mouse spinal neurons.Brain Res. 1999 Dec 4;849(1-2):162-8. doi: 10.1016/s0006-8993(99)02060-0. Brain Res. 1999. PMID: 10592298
-
The contribution of resurgent sodium current to high-frequency firing in Purkinje neurons: an experimental and modeling study.J Neurosci. 2003 Jun 15;23(12):4899-912. doi: 10.1523/JNEUROSCI.23-12-04899.2003. J Neurosci. 2003. PMID: 12832512 Free PMC article.
-
Allelic mutations of the sodium channel SCN8A reveal multiple cellular and physiological functions.Genetica. 2004 Sep;122(1):37-45. doi: 10.1007/s10709-004-1441-9. Genetica. 2004. PMID: 15619959 Review.
-
Sodium channels and neurological disease: insights from Scn8a mutations in the mouse.Neuroscientist. 2001 Apr;7(2):136-45. doi: 10.1177/107385840100700208. Neuroscientist. 2001. PMID: 11496924 Review.
Cited by
-
Ionic mechanisms maintaining action potential conduction velocity at high firing frequencies in an unmyelinated axon.Physiol Rep. 2016 May;4(10):e12814. doi: 10.14814/phy2.12814. Physiol Rep. 2016. PMID: 27225630 Free PMC article.
-
Cav1.3 Channels as Key Regulators of Neuron-Like Firings and Catecholamine Release in Chromaffin Cells.Curr Mol Pharmacol. 2015;8(2):149-61. doi: 10.2174/1874467208666150507105443. Curr Mol Pharmacol. 2015. PMID: 25966692 Free PMC article. Review.
-
Voltage-gated sodium channels: biophysics, pharmacology, and related channelopathies.Front Pharmacol. 2012 Jul 11;3:124. doi: 10.3389/fphar.2012.00124. eCollection 2012. Front Pharmacol. 2012. PMID: 22798951 Free PMC article.
-
Differences in spike generation instead of synaptic inputs determine the feature selectivity of two retinal cell types.Neuron. 2022 Jul 6;110(13):2110-2123.e4. doi: 10.1016/j.neuron.2022.04.012. Epub 2022 May 3. Neuron. 2022. PMID: 35508174 Free PMC article.
-
Structure of human NaV1.6 channel reveals Na+ selectivity and pore blockade by 4,9-anhydro-tetrodotoxin.Nat Commun. 2023 Feb 23;14(1):1030. doi: 10.1038/s41467-023-36766-9. Nat Commun. 2023. PMID: 36823201 Free PMC article.
References
-
- Auld VJ, Goldin AL, Krafte DS, Marshall J, Dunn JM, Catterall WA, Lester HA, Davidson N, Dunn RJ. A rat brain Na+ channel α subunit with novel gating properties. Neuron. 1988;1:449–461. - PubMed
-
- Black JA, Yokoyama S, Higashida H, Ransom BR, Waxman SG. Sodium channel mRNAs I, II, and III in the CNS: cell-specific expression. Mol Brain Res. 1994;22:275–289. - PubMed
-
- Burgess DL, Kohrman DC, Galt J, Plummer NW, Jones JM, Spear B, Meisler MH. Mutation of a new sodium channel gene, Scn8a, in the mouse mutant “motor endplate disease.”. Nat Genet. 1995;10:461–465. - PubMed
-
- Catterall WA. Cellular and molecular biology of voltage-gated sodium channels. Physiol Rev. 1992;72:S15–S48. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases