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. 1998 Jul 15;333 ( Pt 2)(Pt 2):285-90.
doi: 10.1042/bj3330285.

Identification and purification of the reconstitutively active glutamine carrier from rat kidney mitochondria

C Indiveri  1 , G Abruzzo, I Stipani, F Palmieri
Affiliations

Identification and purification of the reconstitutively active glutamine carrier from rat kidney mitochondria

C Indiveri et al. Biochem J. .

Abstract

The glutamine carrier from rat kidney mitochondria, solubilized in dodecyl octaoxyethylene ether (C12E8) and partly purified on hydroxyapatite, was identified and completely purified by Celite chromatography. On SDS/PAGE, the purified glutamine carrier consisted of a single protein band with an apparent molecular mass of 41.5 kDa. When reconstituted into liposomes, the glutamine carrier catalysed both the unidirectional flux of glutamine and the glutamine/glutamine countertransport, which were completely inhibitable by a mixture of pyridoxal 5'-phosphate and N-ethylmaleimide. The carrier protein was purified 474-fold with a recovery of 58% and a protein yield of 0.12% with respect to the mitochondrial extract. The glutamine carrier-mediated transport is quite specific for l-glutamine. l-Asparagine is the only other amino acid that is efficiently transported by the reconstituted carrier protein. d-Glutamine, l-glutamate and l-aspartate are very poor substrates. The transport activity was inhibited by several thiol-group and amino-group reagents.

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