PU.1 and hematopoiesis: lessons learned from gene targeting experiments
- PMID: 9618756
- DOI: 10.1006/smim.1998.0112
PU.1 and hematopoiesis: lessons learned from gene targeting experiments
Abstract
The advent of gene targeting technology in mouse embryonic stem cells has revolutionized the study of the development of organ systems in mammals. Hematopoietic transcription factors play a critical role in blood cell development. Targeted mutagenesis of the murine PU.1 locus has revealed the pivotal role this protein plays in blood cell differentiation at all stages of hematopoiesis (yolk sac, fetal liver and bone marrow). PU.1 has been disrupted by two independent research groups and both strains of PU.1-deficient mice exhibit abnormalities in B cell, T cell, monocyte and neutrophil development. The independent mutations have yielded some differences in phenotype suggesting that the different strategies for gene targeting have resulted in the beginning of an 'allelic series' at the PU.1 locus. Both strains of PU.1-/- mice provide exciting reagents for future study of the role of this factor in blood lineage specification.
Similar articles
-
Visualizing PU.1 activity during hematopoiesis.Exp Hematol. 2005 Apr;33(4):395-402. doi: 10.1016/j.exphem.2004.12.010. Exp Hematol. 2005. PMID: 15781329
-
Role of PU.1 in hematopoiesis.Stem Cells. 1998;16(1):25-37. doi: 10.1002/stem.160025. Stem Cells. 1998. PMID: 9474745 Review.
-
Osteopetrosis in mice lacking haematopoietic transcription factor PU.1.Nature. 1997 Mar 6;386(6620):81-4. doi: 10.1038/386081a0. Nature. 1997. PMID: 9052784
-
PU.1: a crucial and versatile player in hematopoiesis and leukemia.Int J Biochem Cell Biol. 2008;40(1):22-7. doi: 10.1016/j.biocel.2007.01.026. Epub 2007 Feb 4. Int J Biochem Cell Biol. 2008. PMID: 17374502 Review.
-
The role of Ets family transcription factor PU.1 in hematopoietic cell differentiation, proliferation and apoptosis.Cell Death Differ. 1999 Jul;6(7):599-608. doi: 10.1038/sj.cdd.4400534. Cell Death Differ. 1999. PMID: 10453070 Review.
Cited by
-
The Roles of IRF-8 in Regulating IL-9-Mediated Immunologic Mechanisms in the Development of DLBCL: A State-of-the-Art Literature Review.Front Oncol. 2022 Feb 8;12:817069. doi: 10.3389/fonc.2022.817069. eCollection 2022. Front Oncol. 2022. PMID: 35211408 Free PMC article. Review.
-
Trophic macrophages in development and disease.Nat Rev Immunol. 2009 Apr;9(4):259-70. doi: 10.1038/nri2528. Nat Rev Immunol. 2009. PMID: 19282852 Free PMC article. Review.
-
The transcription factor PU.1, necessary for B-cell development is expressed in lymphocyte predominance, but not classical Hodgkin's disease.Am J Pathol. 2001 Nov;159(5):1807-14. doi: 10.1016/S0002-9440(10)63027-1. Am J Pathol. 2001. PMID: 11696441 Free PMC article.
-
PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis.J Cell Mol Med. 2009 Nov-Dec;13(11-12):4349-63. doi: 10.1111/j.1582-4934.2009.00757.x. Epub 2009 Apr 6. J Cell Mol Med. 2009. PMID: 19382896 Free PMC article. Review.
-
Functional cross-antagonism between transcription factors FLI-1 and EKLF.Mol Cell Biol. 2003 Feb;23(4):1390-402. doi: 10.1128/MCB.23.4.1390-1402.2003. Mol Cell Biol. 2003. PMID: 12556498 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
