Biophysical compensation mechanisms buffering E. coli protein-nucleic acid interactions against changing environments
- PMID: 9612084
- DOI: 10.1016/s0968-0004(98)01207-9
Biophysical compensation mechanisms buffering E. coli protein-nucleic acid interactions against changing environments
Abstract
Escherichia coli adapts to changes in growth osmolarity of at least 100-fold by making large changes in the amounts of intracellular water and solutes, including cytoplasmic K+. A wide range of in vitro salt, solute and biopolymer concentrations should therefore be considered 'physiological'. Paradoxically, these large, osmotically induced changes in cytoplasmic K+ concentration do not greatly affect the equilibria and kinetics of cytoplasmic protein-nucleic acid interactions. Biophysical effects resulting from changes in the amount of cytoplasmic water (such as macromolecular crowding) and in the concentrations of other cytoplasmic solutes appear to compensate for the effects of changes in cytoplasmic K+ concentration and thereby maintain protein-nucleic acid equilibria and kinetics in the range required for in vivo function.
Similar articles
-
Large changes in cytoplasmic biopolymer concentration with osmolality indicate that macromolecular crowding may regulate protein-DNA interactions and growth rate in osmotically stressed Escherichia coli K-12.J Mol Recognit. 2004 Sep-Oct;17(5):488-96. doi: 10.1002/jmr.695. J Mol Recognit. 2004. PMID: 15362109 Review.
-
Compensating effects of opposing changes in putrescine (2+) and K+ concentrations on lac repressor-lac operator binding: in vitro thermodynamic analysis and in vivo relevance.J Mol Biol. 1996 Apr 26;258(1):25-36. doi: 10.1006/jmbi.1996.0231. J Mol Biol. 1996. PMID: 8613989
-
Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies.Methods Enzymol. 2007;428:487-504. doi: 10.1016/S0076-6879(07)28027-9. Methods Enzymol. 2007. PMID: 17875435 Review.
-
Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine.Biochemistry. 2003 Nov 4;42(43):12596-609. doi: 10.1021/bi0347297. Biochemistry. 2003. PMID: 14580206
-
Variability of the intracellular ionic environment of Escherichia coli. Differences between in vitro and in vivo effects of ion concentrations on protein-DNA interactions and gene expression.J Biol Chem. 1987 May 25;262(15):7157-64. J Biol Chem. 1987. PMID: 3108249
Cited by
-
From "simple" DNA-protein interactions to the macromolecular machines of gene expression.Annu Rev Biophys Biomol Struct. 2007;36:79-105. doi: 10.1146/annurev.biophys.34.040204.144521. Annu Rev Biophys Biomol Struct. 2007. PMID: 17477836 Free PMC article. Review.
-
Reconstitution and organization of Escherichia coli proto-ring elements (FtsZ and FtsA) inside giant unilamellar vesicles obtained from bacterial inner membranes.J Biol Chem. 2011 Apr 1;286(13):11236-41. doi: 10.1074/jbc.M110.194365. Epub 2011 Jan 21. J Biol Chem. 2011. PMID: 21257762 Free PMC article.
-
Dissociation and re-association of RNA polymerase with DNA during osmotic stress response in Escherichia coli.Nucleic Acids Res. 2013 Jan 7;41(1):315-26. doi: 10.1093/nar/gks988. Epub 2012 Oct 23. Nucleic Acids Res. 2013. PMID: 23093594 Free PMC article.
-
In Vivo Titration of Folate Pathway Enzymes.Appl Environ Microbiol. 2018 Sep 17;84(19):e01139-18. doi: 10.1128/AEM.01139-18. Print 2018 Oct 1. Appl Environ Microbiol. 2018. PMID: 30030232 Free PMC article.
-
Molecular crowding enhances native structure and stability of alpha/beta protein flavodoxin.Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18976-81. doi: 10.1073/pnas.0705127104. Epub 2007 Nov 16. Proc Natl Acad Sci U S A. 2007. PMID: 18024596 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
