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. 1998 Apr;42(4):971-3.
doi: 10.1128/AAC.42.4.971.

Human cytomegalovirus mutant with sequence-dependent resistance to the phosphorothioate oligonucleotide fomivirsen (ISIS 2922)

G B Mulamba  1 , A Hu, R F Azad, K P Anderson, D M Coen
Affiliations

Human cytomegalovirus mutant with sequence-dependent resistance to the phosphorothioate oligonucleotide fomivirsen (ISIS 2922)

G B Mulamba et al. Antimicrob Agents Chemother. 1998 Apr.

Abstract

A human cytomegalovirus mutant that was isolated for resistance (10-fold) to the antisense oligonucleotide fomivirsen (ISIS 2922) exhibited cross-resistance to a modified derivative of fomivirsen with an identical base sequence but little or no resistance to an oligonucleotide with an unrelated sequence. No changes in the mutant's DNA corresponding to the fomivirsen target sequence were found.

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Figures

FIG. 1
FIG. 1
Procedures used to isolate a mutant resistant to fomivirsen. HFF, human foreskin fibroblasts; M.O.I., multiplicity of infection.
FIG. 2
FIG. 2
Susceptibilities to phosphorothioate oligonucleotides. Plaque reduction assays were performed as described in the text. Results presented are the averages of two separate experiments, each performed in duplicate. Similar results were obtained in a third experiment, in which different drug concentrations were used. Error bars represent standard errors of the means. Error bars are not presented for standard errors of the means that were too small to be visible on the plots.
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