Ethanol inhibition of NMDA currents in acutely dissociated medial septum/diagonal band neurons from ethanol dependent rats
- PMID: 9519248
- DOI: 10.1016/s0006-8993(97)01001-9
Ethanol inhibition of NMDA currents in acutely dissociated medial septum/diagonal band neurons from ethanol dependent rats
Abstract
The effect of acutely applied ethanol and the impact of chronic ethanol treatment, sufficient to induce tolerance and physical dependence, on N-methyl-D-aspartate (NMDA) receptor function were studied in acutely isolated neurons from the medial septum/diagonal band (MS/DB) of adult rats using whole cell, patch-clamp electrophysiology. There was a small positive correlation for capacitance and current amplitude activated by 100 microM NMDA for all groups. Also, cell membrane capacitance was significantly smaller for Ethanol Dependent (approximately 80-84%) than either Naive or Control cells. Therefore NMDA-activated responses were normalized for capacitance (current density, pA/pF) across all three groups. NMDA-activated (30-1000 microM) responses were significantly larger in cells from Control and Ethanol Dependent rats relative to those from Naives. In addition, estimated maximal responses were significantly larger for Ethanol Dependent cells, compared to either Control or Naive, respectively, while EC50s and slopes were not significantly different. Acute 60 mM ethanol significantly inhibited responses to 100 microM NMDA in all three groups, however, mean ethanol inhibition was 12-25% smaller after ethanol dependence. There was no evidence of acute tolerance to ethanol inhibition for any group, but examination of patterns of inhibition for individual neurons showed a few cells were resistant to ethanol or exhibited progressive loss of ethanol inhibition. These results suggest that NMDA receptor function in acutely isolated MS/DB neurons is increased following in vivo chronic ethanol treatment, and shows resistance to acute ethanol inhibition suggesting NMDA receptor-mediated cellular tolerance.
Similar articles
-
Sustained ethanol inhibition of native AMPA receptors on medial septum/diagonal band (MS/DB) neurons.Br J Pharmacol. 2000 Jan;129(1):87-94. doi: 10.1038/sj.bjp.0703039. Br J Pharmacol. 2000. PMID: 10694206 Free PMC article.
-
Interaction of ethanol and allosteric modulators with GABAA-activated currents in adult medial septum/diagonal band neurons.Brain Res. 1994 Jan 28;635(1-2):283-92. doi: 10.1016/0006-8993(94)91449-4. Brain Res. 1994. PMID: 8173965
-
Ethanol inhibition of reduced frequency-dependent rundown of calcium currents in acutely dissociated MS/nDB neurons from chronic in vivo lead-exposed adult rats.Neurotoxicology. 1997;18(1):179-90. Neurotoxicology. 1997. PMID: 9216000
-
Lanthanum and zinc sensitivity of GABAA-activated currents in adult medial septum/diagonal band neurons from ethanol dependent rats.Brain Res. 1996 May 13;720(1-2):101-10. doi: 10.1016/0006-8993(96)00154-0. Brain Res. 1996. PMID: 8782902
-
Comparison of effect of ethanol on N-methyl-D-aspartate- and GABA-gated currents from acutely dissociated neurons: absence of regional differences in sensitivity to ethanol.J Pharmacol Exp Ther. 2003 Jan;304(1):192-9. doi: 10.1124/jpet.102.041590. J Pharmacol Exp Ther. 2003. PMID: 12490591
Cited by
-
Synaptic Effects Induced by Alcohol.Curr Top Behav Neurosci. 2023 Feb 11:10.1007/7854_2022_412. doi: 10.1007/7854_2022_412. Online ahead of print. Curr Top Behav Neurosci. 2023. PMID: 36765015 Free PMC article.
-
Glutamate plasticity in the drunken amygdala: the making of an anxious synapse.Int Rev Neurobiol. 2010;91:205-33. doi: 10.1016/S0074-7742(10)91007-6. Int Rev Neurobiol. 2010. PMID: 20813244 Free PMC article. Review.
-
Chronic Ethanol Exposure Enhances Facial Stimulation-Evoked Mossy Fiber-Granule Cell Synaptic Transmission via GluN2A Receptors in the Mouse Cerebellar Cortex.Front Syst Neurosci. 2021 Aug 2;15:657884. doi: 10.3389/fnsys.2021.657884. eCollection 2021. Front Syst Neurosci. 2021. PMID: 34408633 Free PMC article.
-
Synaptic effects induced by alcohol.Curr Top Behav Neurosci. 2013;13:31-86. doi: 10.1007/7854_2011_143. Curr Top Behav Neurosci. 2013. PMID: 21786203 Free PMC article. Review.
-
Sustained ethanol inhibition of native AMPA receptors on medial septum/diagonal band (MS/DB) neurons.Br J Pharmacol. 2000 Jan;129(1):87-94. doi: 10.1038/sj.bjp.0703039. Br J Pharmacol. 2000. PMID: 10694206 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous