Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays
- PMID: 9447592
- DOI: 10.1038/nbt0198-45
Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays
Abstract
We have used high-density oligonucleotide probe arrays (chips) for bacterial transcript imaging. We designed a chip containing probes representing 106 Hemophilus influenzae genes and 100 Streptococcus pneumoniae genes. The apparent lack of polyadenylated transcripts excludes enrichment of mRNA by affinity purification and we thus used total, chemically biotinylated RNA as hybridization probe. We show that hybridization of Streptococcus RNA to a chip allows simultaneous quantification of the transcript levels. The sensitivity was found to be in the range of one to five transcripts per cell. The quantitative chip results were in good agreement with conventional Northern blot analysis of selected genes. This technology allows simultaneous and quantitative measurement of the transcriptional activity of entire bacterial genomes on a single oligonucleotide probe array.
Comment in
-
A panoramic view of bacterial transcription.Nat Biotechnol. 1998 Jan;16(1):23. doi: 10.1038/nbt0198-23. Nat Biotechnol. 1998. PMID: 9447586 No abstract available.
Similar articles
-
A panoramic view of bacterial transcription.Nat Biotechnol. 1998 Jan;16(1):23. doi: 10.1038/nbt0198-23. Nat Biotechnol. 1998. PMID: 9447586 No abstract available.
-
Nucleotide sequence changes between Streptococcus pneumoniae R6 and D39 strains determined by an oligonucleotide hybridization DNA sequencing technology.J Microbiol Methods. 2007 Jul;70(1):65-74. doi: 10.1016/j.mimet.2007.03.009. Epub 2007 Mar 30. J Microbiol Methods. 2007. PMID: 17477996
-
Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression.BMC Biotechnol. 2004 Jun 10;4:12. doi: 10.1186/1472-6750-4-12. BMC Biotechnol. 2004. PMID: 15191615 Free PMC article.
-
[A novel oligonucleotide arrays-based multiplex amplifiable probe hybridization technology].Yi Chuan Xue Bao. 2004 Feb;31(2):119-24. Yi Chuan Xue Bao. 2004. PMID: 15473300 Chinese.
-
Using oligonucleotide probe arrays to access genetic diversity.Biotechniques. 1995 Sep;19(3):442-7. Biotechniques. 1995. PMID: 7495558 Review.
Cited by
-
Novel concept microarray enabling PCR and multistep reactions through pipette-free aperture-to-aperture parallel transfer.BMC Biotechnol. 2010 Oct 6;10:71. doi: 10.1186/1472-6750-10-71. BMC Biotechnol. 2010. PMID: 20923572 Free PMC article.
-
Combined transcriptome and proteome analysis as a powerful approach to study genes under glucose repression in Bacillus subtilis.Nucleic Acids Res. 2001 Feb 1;29(3):683-92. doi: 10.1093/nar/29.3.683. Nucleic Acids Res. 2001. PMID: 11160890 Free PMC article.
-
Microarray techniques in pathology: tool or toy?Mol Pathol. 2000 Dec;53(6):289-94. doi: 10.1136/mp.53.6.289. Mol Pathol. 2000. PMID: 11193046 Free PMC article. Review.
-
Global transcriptional analysis of clpP mutations of type 2 Streptococcus pneumoniae and their effects on physiology and virulence.J Bacteriol. 2002 Jul;184(13):3508-20. doi: 10.1128/JB.184.13.3508-3520.2002. J Bacteriol. 2002. PMID: 12057945 Free PMC article.
-
Mosaic genes and mosaic chromosomes: intra- and interspecies genomic variation of Streptococcus pneumoniae.Infect Immun. 2001 Apr;69(4):2477-86. doi: 10.1128/IAI.69.4.2477-2486.2001. Infect Immun. 2001. PMID: 11254610 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
