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. 1998 Jan 15;58(2):296-301.

CD4+ T cells from peripheral blood of a melanoma patient recognize peptides derived from nonmutated tyrosinase

H Kobayashi  1 , T Kokubo, K Sato, S Kimura, K Asano, H Takahashi, H Iizuka, N Miyokawa, M Katagiri
Affiliations
  • PMID: 9443408

CD4+ T cells from peripheral blood of a melanoma patient recognize peptides derived from nonmutated tyrosinase

H Kobayashi et al. Cancer Res. .

Abstract

Tyrosinase is an antigen that is expressed by normal melanocytes as well as melanoma cells, against which responses by autologous T cells have been detected. Although CD4+ T cells play an important role in tumor immunity in animal tumor models, little information about CD4+ T-cell immunity against human tumors exists. Here, we report that CD4+ T cells from the peripheral blood of a patient with melanoma respond to synthetic peptides derived from nonmutated tyrosinase. T-cell clones were generated that recognized the tyrosinase p386-406 peptide when it was presented by the HLA-DR15 (DRB1*1501) molecule. The CD4+ T-cell clone also recognized autologous EBV-transformed B-lymphoblastoid cell lines that had been pulsed with the lysate of melanoma cells. The synthetic tyrosinase p386-406 peptide was capable of binding to HLA-DR15 (DRB1*1501) molecules on cell surface of DR15 homozygous cells. Thus, the finding that nonmutated tyrosinase peptides are immunogenic in a melanoma patient may provide the basis for the development of cancer immunotherapy, based on knowledge of synthetic tumor-associated peptide antigens.

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