Inhibition of tumor necrosis factor (TNF-alpha)-mediated apoptosis by hepatitis C virus core protein
- PMID: 9442069
- DOI: 10.1074/jbc.273.4.2256
Inhibition of tumor necrosis factor (TNF-alpha)-mediated apoptosis by hepatitis C virus core protein
Abstract
Hepatitis C virus (HCV) putative core protein has displayed many intriguing biological properties. Since tumor necrosis factor (TNF) plays an important role in controlling viral infection, in this study the effect of the core protein was investigated on the TNF-alpha induced apoptosis of human breast carcinoma cells (MCF7). HCV core protein when expressed inhibited TNF-alpha-induced apoptotic cell death unlike the control MCF7 cells, as determined by cell viability and DNA fragmentation analysis. Additionally, HCV core protein blocked the TNF-induced proteolytic cleavage of the death substrate poly(ADP-ribose) polymerase from its native 116-kDa protein to the characteristic 85-kDa polypeptide. Results from this study suggest that the HCV core protein plays a role in the inhibition of TNF-alpha-mediated cell death. Thus, the ability of core protein to inhibit the TNF-mediated apoptotic signaling pathway may provide a selective advantage for HCV replication, allowing for evasion of host antiviral defense mechanisms.
Similar articles
-
Hepatitis C virus core protein inhibits tumor necrosis factor alpha-mediated apoptosis by a protective effect involving cellular FLICE inhibitory protein.J Virol. 2006 May;80(9):4372-9. doi: 10.1128/JVI.80.9.4372-4379.2006. J Virol. 2006. PMID: 16611896 Free PMC article.
-
Evasion of TNF-α-mediated apoptosis by hepatitis C virus.Methods Mol Biol. 2014;1155:125-32. doi: 10.1007/978-1-4939-0669-7_11. Methods Mol Biol. 2014. PMID: 24788178
-
Hepatitis C virus core protein potentiates TNF-alpha-induced NF-kappaB activation through TRAF2-IKKbeta-dependent pathway.Biochem Biophys Res Commun. 2001 Jun 1;284(1):15-9. doi: 10.1006/bbrc.2001.4936. Biochem Biophys Res Commun. 2001. PMID: 11374864
-
Hepatitis C virus core protein binds to the cytoplasmic domain of tumor necrosis factor (TNF) receptor 1 and enhances TNF-induced apoptosis.J Virol. 1998 May;72(5):3691-7. doi: 10.1128/JVI.72.5.3691-3697.1998. J Virol. 1998. PMID: 9557650 Free PMC article.
-
Cleavage of Poly(ADP-ribose) polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis.Exp Cell Res. 2000 Feb 25;255(1):125-32. doi: 10.1006/excr.1999.4796. Exp Cell Res. 2000. PMID: 10666341
Cited by
-
Hepatitis C virus infection: establishment of chronicity and liver disease progression.EXCLI J. 2014 Aug 27;13:977-96. eCollection 2014. EXCLI J. 2014. PMID: 26417315 Free PMC article. Review.
-
Hepatitis C virus Core overcomes all-trans retinoic acid-induced apoptosis in human hepatoma cells by inhibiting p14 expression via DNA methylation.Oncotarget. 2017 Aug 18;8(49):85584-85598. doi: 10.18632/oncotarget.20337. eCollection 2017 Oct 17. Oncotarget. 2017. PMID: 29156743 Free PMC article.
-
Oxidative stress and apoptosis in hepatitis C: the core issue.J Gastroenterol. 2006 Mar;41(3):292-4. doi: 10.1007/s00535-006-1786-1. J Gastroenterol. 2006. PMID: 16699868 No abstract available.
-
Hepatitis C virus core protein-induced loss of LZIP function correlates with cellular transformation.EMBO J. 2000 Feb 15;19(4):729-40. doi: 10.1093/emboj/19.4.729. EMBO J. 2000. PMID: 10675342 Free PMC article.
-
Non-encapsidation activities of the capsid proteins of positive-strand RNA viruses.Virology. 2013 Nov;446(1-2):123-32. doi: 10.1016/j.virol.2013.07.023. Epub 2013 Aug 27. Virology. 2013. PMID: 24074574 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
