doi: 10.1038/34593.
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution
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- PMID: 9440688
- DOI: 10.1038/34593
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Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution
Nature.
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Abstract
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.
Comment in
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A mechanism for all polymerases.Nature. 1998 Jan 15;391(6664):231-2. doi: 10.1038/34542. Nature. 1998. PMID: 9440683 No abstract available.
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