Regulation of cell-cell adhesion of MDCK cells by Cdc42 and Rac1 small GTPases
- PMID: 9388496
- DOI: 10.1006/bbrc.1997.7675
Regulation of cell-cell adhesion of MDCK cells by Cdc42 and Rac1 small GTPases
Abstract
Rac1, a member of the Rho small GTPases family, has recently been shown to be involved in the regulation of cell-cell adhesion mediated by cadherin. Here we showed that Cdc42, another member of Rho family, accumulated at cell-cell contact sites. Microinjection of Rho GDI, a negative regulator of the Rho family members, into Madin-Darby canine kidney (MDCK) cells resulted in perturbation of epithelial cell morphology and of cell-cell and cell-substratum adhesions, and comicroinjection of dominant active Cdc42 or Rac1 reversed the action of Rho GDI, suggesting that the active form of Cdc42 or Rac1 is required for maintaining the cell-cell and cell-substratum adhesions. These observations suggest that Cdc42, in addition to Rac1, can regulate the cell-cell adhesion.
Similar articles
-
Involvement of Cdc42 small G protein in cell-cell adhesion, migration and morphology of MDCK cells.Oncogene. 1999 Jul 8;18(27):3996-4006. doi: 10.1038/sj.onc.1202773. Oncogene. 1999. PMID: 10435623
-
A balance of signaling by Rho family small GTPases RhoA, Rac1 and Cdc42 coordinates cytoskeletal morphology but not cell survival.Oncogene. 1999 Jan 7;18(1):47-57. doi: 10.1038/sj.onc.1202262. Oncogene. 1999. PMID: 9926919
-
Cdc42, Rac1, and their effector IQGAP1 as molecular switches for cadherin-mediated cell-cell adhesion.Biochem Biophys Res Commun. 1999 Aug 19;262(1):1-6. doi: 10.1006/bbrc.1999.1122. Biochem Biophys Res Commun. 1999. PMID: 10448058 Review.
-
Involvement of IQGAP1, an effector of Rac1 and Cdc42 GTPases, in cell-cell dissociation during cell scattering.Mol Cell Biol. 2001 Mar;21(6):2165-83. doi: 10.1128/MCB.21.6.2165-2183.2001. Mol Cell Biol. 2001. PMID: 11238950 Free PMC article.
-
Rho-family GTPases in cadherin-mediated cell-cell adhesion.Nat Rev Mol Cell Biol. 2001 Dec;2(12):887-97. doi: 10.1038/35103068. Nat Rev Mol Cell Biol. 2001. PMID: 11733768 Review.
Cited by
-
Mutant B-RAF regulates a Rac-dependent cadherin switch in melanoma.Oncogene. 2013 Oct;32(40):4836-44. doi: 10.1038/onc.2012.492. Epub 2012 Dec 3. Oncogene. 2013. PMID: 23208503 Free PMC article.
-
Extracellular matrix, junctional integrity and matrix metalloproteinase interactions in endothelial permeability regulation.J Anat. 2002 Jun;200(6):561-74. doi: 10.1046/j.1469-7580.2002.00057.x. J Anat. 2002. PMID: 12162724 Free PMC article. Review.
-
Rac1 activation inhibits E-cadherin-mediated adherens junctions via binding to IQGAP1 in pancreatic carcinoma cells.Cell Commun Signal. 2009 Sep 8;7:23. doi: 10.1186/1478-811X-7-23. Cell Commun Signal. 2009. PMID: 19737400 Free PMC article.
-
Activation of cdc42, rac, PAK, and rho-kinase in response to hepatocyte growth factor differentially regulates epithelial cell colony spreading and dissociation.Mol Biol Cell. 2000 May;11(5):1709-25. doi: 10.1091/mbc.11.5.1709. Mol Biol Cell. 2000. PMID: 10793146 Free PMC article.
-
p120 catenin regulates the actin cytoskeleton via Rho family GTPases.J Cell Biol. 2000 Aug 7;150(3):567-80. doi: 10.1083/jcb.150.3.567. J Cell Biol. 2000. PMID: 10931868 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
