Annealing vs. invasion in phage lambda recombination
- PMID: 9383045
- PMCID: PMC1208271
- DOI: 10.1093/genetics/147.3.961
Annealing vs. invasion in phage lambda recombination
Abstract
Genetic recombination catalyzed by lambda's Red pathway was studied in rec+ and recA mutant bacteria by examining both intracellular lambda DNA and mature progeny particles. Recombination of nonreplicating phage chromosomes was induced by double-strand breaks delivered at unique sites in vivo. In rec+ cells, cutting only one chromosome gave nearly maximal stimulation of recombination; the recombinants formed contained relatively short hybrid regions, suggesting strand invasion. In contrast, in recA mutant cells, cutting the two parental chromosomes at non-allelic sites was required for maximal stimulation; the recombinants formed tended to be hybrid over the entire region between the two cuts, implying strand annealing. We conclude that, in the absence of RecA and the presence of non-allelic DNA ends, the Red pathway of lambda catalyzes recombination primarily by annealing.
Similar articles
-
Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli.mBio. 2016 Sep 13;7(5):e01443-16. doi: 10.1128/mBio.01443-16. mBio. 2016. PMID: 27624131 Free PMC article.
-
Single-strand DNA intermediates in phage lambda's Red recombination pathway.Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2951-6. doi: 10.1073/pnas.94.7.2951. Proc Natl Acad Sci U S A. 1997. PMID: 9096327 Free PMC article.
-
Break-join recombination in phage lambda.Genetics. 1990 Jul;125(3):463-74. doi: 10.1093/genetics/125.3.463. Genetics. 1990. PMID: 2143162 Free PMC article.
-
Recombination in phage lambda: one geneticist's historical perspective.Gene. 1998 Nov 26;223(1-2):95-102. doi: 10.1016/s0378-1119(98)00246-7. Gene. 1998. PMID: 9858698 Review.
-
Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda.Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents. doi: 10.1128/MMBR.63.4.751-813.1999. Microbiol Mol Biol Rev. 1999. PMID: 10585965 Free PMC article. Review.
Cited by
-
Examining a DNA Replication Requirement for Bacteriophage λ Red- and Rac Prophage RecET-Promoted Recombination in Escherichia coli.mBio. 2016 Sep 13;7(5):e01443-16. doi: 10.1128/mBio.01443-16. mBio. 2016. PMID: 27624131 Free PMC article.
-
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes.Nat Methods. 2011 Dec 4;9(1):103-9. doi: 10.1038/nmeth.1803. Nat Methods. 2011. PMID: 22138824
-
Catalysis of strand exchange by the HSV-1 UL12 and ICP8 proteins: potent ICP8 recombinase activity is revealed upon resection of dsDNA substrate by nuclease.J Mol Biol. 2004 Sep 3;342(1):57-71. doi: 10.1016/j.jmb.2004.07.012. J Mol Biol. 2004. PMID: 15313607 Free PMC article.
-
Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells.BMC Mol Biol. 2003 Jan 16;4(1):1. doi: 10.1186/1471-2199-4-1. Print 2003 Jan 16. BMC Mol Biol. 2003. PMID: 12530927 Free PMC article.
-
Oligomeric complexes formed by Redβ single strand annealing protein in its different DNA bound states.Nucleic Acids Res. 2021 Apr 6;49(6):3441-3460. doi: 10.1093/nar/gkab125. Nucleic Acids Res. 2021. PMID: 33693865 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
