The function of the spike protein of mouse hepatitis virus strain A59 can be studied on virus-like particles: cleavage is not required for infectivity
- PMID: 9371603
- PMCID: PMC230247
- DOI: 10.1128/JVI.71.12.9427-9433.1997
The function of the spike protein of mouse hepatitis virus strain A59 can be studied on virus-like particles: cleavage is not required for infectivity
Abstract
The spike protein (S) of the murine coronavirus mouse hepatitis virus strain A59 (MHV-A59) induces both virus-to-cell fusion during infection and syncytium formation. Thus far, only syncytium formation could be studied after transient expression of S. We have recently described a system in which viral infectivity is mimicked by using virus-like particles (VLPs) and reporter defective-interfering (DI) RNAs (E. C. W. Bos, W. Luytjes, H. Van der Meulen, H. K. Koerten, and W. J. M. Spaan, Virology 218:52-60, 1996). Production of VLPs of MHV-A59 was shown to be dependent on the expression of M and E. We now show in several ways that the infectivity of VLPs is dependent on S. Infectivity was lost when spikeless VLPs were produced. Infectivity was blocked upon treatment of the VLPs with MHV-A59-neutralizing anti-S monoclonal antibody (MAb) A2.3 but not with nonneutralizing anti-S MAb A1.4. When the target cells were incubated with antireceptor MAb CC1, which blocks MHV-A59 infection, VLPs did not infect the target cells. Thus, S-mediated VLP infectivity resembles MHV-A59 infectivity. The system can be used to identify domains in S that are essential for infectivity. As a first application, we investigated the requirements of cleavage of S for the infectivity of MHV-A59. We inserted three mutant S proteins that were previously shown to be uncleaved (E. C. W. Bos, L. Heijnen, W. Luytjes, and W. J. M. Spaan, Virology 214:453-463, 1995) into the VLPs. Here we show that cleavage of the spike protein of MHV-A59 is not required for infectivity.
Similar articles
-
The murine coronavirus mouse hepatitis virus strain A59 from persistently infected murine cells exhibits an extended host range.J Virol. 1997 Dec;71(12):9499-507. doi: 10.1128/JVI.71.12.9499-9507.1997. J Virol. 1997. PMID: 9371612 Free PMC article.
-
Identification of H209 as Essential for pH 8-Triggered Receptor-Independent Syncytium Formation by S Protein of Mouse Hepatitis Virus A59.J Virol. 2018 May 14;92(11):e00209-18. doi: 10.1128/JVI.00209-18. Print 2018 Jun 1. J Virol. 2018. PMID: 29514915 Free PMC article.
-
Amino acid substitutions and an insertion in the spike glycoprotein extend the host range of the murine coronavirus MHV-A59.Virology. 2004 Jul 1;324(2):510-24. doi: 10.1016/j.virol.2004.04.005. Virology. 2004. PMID: 15207636 Free PMC article.
-
Murine coronavirus neuropathogenesis: determinants of virulence.J Neurovirol. 2010 Nov;16(6):427-34. doi: 10.3109/13550284.2010.529238. Epub 2010 Nov 12. J Neurovirol. 2010. PMID: 21073281 Free PMC article. Review.
-
Coronavirus spike proteins in viral entry and pathogenesis.Virology. 2001 Jan 20;279(2):371-4. doi: 10.1006/viro.2000.0757. Virology. 2001. PMID: 11162792 Free PMC article. Review. No abstract available.
Cited by
-
Genetic Engineering Systems to Study Human Viral Pathogens from the Coronaviridae Family.Mol Biol. 2022;56(1):72-89. doi: 10.1134/S0026893322010022. Epub 2022 Feb 12. Mol Biol. 2022. PMID: 35194246 Free PMC article.
-
Review: A systematic review of virus-like particles of coronavirus: Assembly, generation, chimerism and their application in basic research and in the clinic.Int J Biol Macromol. 2022 Mar 1;200:487-497. doi: 10.1016/j.ijbiomac.2022.01.108. Epub 2022 Jan 20. Int J Biol Macromol. 2022. PMID: 35065135 Free PMC article.
-
Genotyping coronaviruses associated with feline infectious peritonitis.J Gen Virol. 2015 Jun;96(Pt 6):1358-1368. doi: 10.1099/vir.0.000084. Epub 2015 Feb 9. J Gen Virol. 2015. PMID: 25667330 Free PMC article.
-
Identification of novel functional regions within the spike glycoprotein of MHV-A59 based on a bioinformatics approach.Virus Res. 2014 Aug 30;189:177-88. doi: 10.1016/j.virusres.2014.05.023. Epub 2014 Jun 5. Virus Res. 2014. PMID: 24910120 Free PMC article.
-
Identification and characterization of a proteolytically primed form of the murine coronavirus spike proteins after fusion with the target cell.J Virol. 2014 May;88(9):4943-52. doi: 10.1128/JVI.03451-13. Epub 2014 Feb 19. J Virol. 2014. PMID: 24554652 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials