High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria
- PMID: 9369223
- DOI: 10.1016/s0014-5793(97)01159-9
High protonic potential actuates a mechanism of production of reactive oxygen species in mitochondria
Abstract
Formation of H2O2 has been studied in rat heart mitochondria, pretreated with H2O2 and aminotriazole to lower their antioxidant capacity. It is shown that the rate of H2O2 formation by mitochondria oxidizing 6 mM succinate is inhibited by a protonophorous uncoupler, ADP and phosphate, malonate, rotenone and myxothiazol, and is stimulated by antimycin A. The effect of ADP is abolished by carboxyatractylate and oligomycin. Addition of uncoupler after rotenone induces further inhibition of H2O2 production. Inhibition of H2O2 formation by uncoupler, malonate and ADP+Pi is shown to be proportional to the delta psi decrease by these compounds. A threshold delta psi value is found, above which a very strong increase in H2O2 production takes place. This threshold slightly exceeds the state 3 delta psi level. The data obtained are in line with the concept [Skulachev, V.P., Q. Rev. Biophys. 29 (1996), 169-2021 that a high proton motive force in state 4 is potentially dangerous for the cell due to an increase in the probability of superoxide formation.
Similar articles
-
Hydrogen peroxide generation by higher plant mitochondria oxidizing complex I or complex II substrates.FEBS Lett. 1999 May 28;451(3):347-50. doi: 10.1016/s0014-5793(99)00616-x. FEBS Lett. 1999. PMID: 10371218
-
Stability of membrane potential in heart mitochondria: single mitochondrion imaging.Biochem Biophys Res Commun. 2006 Jun 16;344(4):1094-101. doi: 10.1016/j.bbrc.2006.03.233. Epub 2006 Apr 19. Biochem Biophys Res Commun. 2006. PMID: 16647039
-
Fatty acids as natural uncouplers preventing generation of O2.- and H2O2 by mitochondria in the resting state.FEBS Lett. 1998 Sep 18;435(2-3):215-8. doi: 10.1016/s0014-5793(98)01073-4. FEBS Lett. 1998. PMID: 9762912
-
Depolarization of in situ mitochondria due to hydrogen peroxide-induced oxidative stress in nerve terminals: inhibition of alpha-ketoglutarate dehydrogenase.J Neurochem. 1999 Jul;73(1):220-8. doi: 10.1046/j.1471-4159.1999.0730220.x. J Neurochem. 1999. PMID: 10386974
-
The production of reactive oxygen species in intact isolated nerve terminals is independent of the mitochondrial membrane potential.Neurochem Res. 2003 Oct;28(10):1575-81. doi: 10.1023/a:1025634728227. Neurochem Res. 2003. PMID: 14570403
Cited by
-
Diabetic Retinopathy: The Role of Mitochondria in the Neural Retina and Microvascular Disease.Antioxidants (Basel). 2020 Sep 23;9(10):905. doi: 10.3390/antiox9100905. Antioxidants (Basel). 2020. PMID: 32977483 Free PMC article. Review.
-
In adipose tissue, increased mitochondrial emission of reactive oxygen species is important for short-term high-fat diet-induced insulin resistance in mice.Diabetologia. 2015 May;58(5):1071-80. doi: 10.1007/s00125-015-3531-x. Epub 2015 Mar 11. Diabetologia. 2015. PMID: 25754553
-
Uncoupling protein-2 expression and effects on mitochondrial membrane potential and oxidant stress in heart tissue.Transl Res. 2012 May;159(5):383-90. doi: 10.1016/j.trsl.2011.11.001. Epub 2011 Dec 2. Transl Res. 2012. PMID: 22500511 Free PMC article.
-
Altered expression of mitochondrial electron transport chain proteins and improved myocardial energetic state during late ischemic preconditioning.Am J Physiol Heart Circ Physiol. 2012 May 15;302(10):H1974-82. doi: 10.1152/ajpheart.00372.2011. Epub 2012 Mar 2. Am J Physiol Heart Circ Physiol. 2012. PMID: 22389388 Free PMC article.
-
The Relationship between Mitochondrial Reactive Oxygen Species Production and Mitochondrial Energetics in Rat Tissues with Different Contents of Reduced Coenzyme Q.Antioxidants (Basel). 2021 Mar 29;10(4):533. doi: 10.3390/antiox10040533. Antioxidants (Basel). 2021. PMID: 33805446 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous
