Studies on the mechanism of DNA linking by Epstein-Barr virus nuclear antigen 1
- PMID: 9368061
- DOI: 10.1074/jbc.272.47.29873
Studies on the mechanism of DNA linking by Epstein-Barr virus nuclear antigen 1
Abstract
Epstein-Barr virus nuclear antigen 1 (EBNA1) can both bind to and link DNA. Dimers of EBNA1 bind specific sites, two clusters of which, the FR and DS, comprise the necessary cis-acting elements of the Epstein-Barr viral origin of plasmid replication. EBNA1-dimers can link FR and DS, looping out the intervening DNA. EBNA1 can also intermolecularly link DNAs to which it binds. Residues of EBNA1 that can mediate linking have been mapped to at least three, non-overlapping domains. These domains, when fused to the dimerization and DNA-binding domain of GAL4, can self-associate and thereby link DNAs bound site specifically by GAL4. Two disparate mechanisms could underlie self-association of linking domains: 1) linking domains could associate with other linking domains directly, or 2) linking domains could associate indirectly by binding to a common nucleic acid intermediate. We have found that EBNA1 can link DNA by each of these mechanisms, however, the linking domains associate directly with a greater apparent affinity than through a nonspecific nucleic acid intermediate.
Similar articles
-
Multiple regions within EBNA1 can link DNAs.J Virol. 1995 Oct;69(10):6199-208. doi: 10.1128/JVI.69.10.6199-6208.1995. J Virol. 1995. PMID: 7666521 Free PMC article.
-
Structural Basis for Cooperative Binding of EBNA1 to the Epstein-Barr Virus Dyad Symmetry Minimal Origin of Replication.J Virol. 2019 Sep 30;93(20):e00487-19. doi: 10.1128/JVI.00487-19. Print 2019 Oct 15. J Virol. 2019. PMID: 31142669 Free PMC article.
-
Stabilization of the EBNA1 protein on the Epstein-Barr virus latent origin of DNA replication by a DNA looping mechanism.J Biol Chem. 1994 Jan 14;269(2):1057-62. J Biol Chem. 1994. PMID: 8288561
-
The 2.2 A structure of a permanganate-sensitive DNA site bound by the Epstein-Barr virus origin binding protein, EBNA1.J Mol Biol. 1998 Dec 18;284(5):1273-8. doi: 10.1006/jmbi.1998.2247. J Mol Biol. 1998. PMID: 9878348 Review.
-
Two steps to binding replication origins? DNA-protein interactions.Curr Biol. 1996 Aug 1;6(8):931-4. doi: 10.1016/s0960-9822(02)00629-2. Curr Biol. 1996. PMID: 8805327 Review.
Cited by
-
Promoter-proximal regulatory elements involved in oriP-EBNA1-independent and -dependent activation of the Epstein-Barr virus C promoter in B-lymphoid cell lines.J Virol. 2001 Jul;75(13):5796-811. doi: 10.1128/JVI.75.13.5796-5811.2001. J Virol. 2001. PMID: 11390581 Free PMC article.
-
The amino terminus of Epstein-Barr Virus (EBV) nuclear antigen 1 contains AT hooks that facilitate the replication and partitioning of latent EBV genomes by tethering them to cellular chromosomes.J Virol. 2004 Nov;78(21):11487-505. doi: 10.1128/JVI.78.21.11487-11505.2004. J Virol. 2004. PMID: 15479791 Free PMC article.
-
The mitotic chromosome binding activity of the papillomavirus E2 protein correlates with interaction with the cellular chromosomal protein, Brd4.J Virol. 2005 Apr;79(8):4806-18. doi: 10.1128/JVI.79.8.4806-4818.2005. J Virol. 2005. PMID: 15795266 Free PMC article.
-
Maintenance of Epstein-Barr virus (EBV) oriP-based episomes requires EBV-encoded nuclear antigen-1 chromosome-binding domains, which can be replaced by high-mobility group-I or histone H1.Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1865-70. doi: 10.1073/pnas.98.4.1865. Epub 2001 Feb 6. Proc Natl Acad Sci U S A. 2001. PMID: 11172042 Free PMC article.
-
The linking regions of EBNA1 are essential for its support of replication and transcription.Mol Cell Biol. 1999 May;19(5):3349-59. doi: 10.1128/MCB.19.5.3349. Mol Cell Biol. 1999. PMID: 10207059 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
