Isolation of disaturated phosphatidylcholine with osmium tetroxide
- PMID: 932560
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Isolation of disaturated phosphatidylcholine with osmium tetroxide
J Lipid Res.
1976 May.
Free article
Abstract
A simple, rapid, inexpensive method for isolating disaturated phosphatidylcholine from adult rat lung has been devised. Total lipids are reacted with osmium tetroxide dissolved in carbon tetrachloride, and the disaturated phosphatidylcholine is isolated on a column of neutral alumina. More than 99% of the fatty acids in the phosphatidylcholine fraction are saturated and 94% of this material migrates as phosphatidylcholine on subsequent thin-layer chromatography.
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