Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr
- PMID: 9308170
- DOI: 10.1099/00221287-143-9-2865
Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr
Abstract
The ndh gene of Escherichia coli encodes the non-proton-translocating NADH dehydrogenase II. Expression of the ndh gene is subject to a complex network of regulatory controls at the transcriptional level. Under anaerobic conditions ndh is repressed by the regulator of fumarate and nitrate reduction (FNR). However, in the absence of FNR, ndh expression is activated by the amino acid response regulator (Arr) during anaerobic growth in rich medium. Expression of the ndh gene varies during the growth cycle in response to the intracellular concentration of the heat-stable DNA-binding protein, Fis. In this work two additional heat-stable proteins, integration host factor (IHF) and the histone-like protein HU were found to interact with the ndh promoter. IHF was shown to bind at three sites centred at +26, -17 and -58 in the ndh promoter (Kd = 10(-8) M), to prevent open-complex formation and to repress ndh transcription in vitro. Studies with an ndh-lacZ fusion confirmed that IHF represses ndh expression in vivo. Two putative binding sites for Arr, which overlap the two FNR boxes in the ndh promoter, were identified. Studies with the FNR-activated and amino-acid-inducible asparaginase II gene (ansB) showed that IHF and a component of the Arr-containing fraction (but not HU) interact with the corresponding ansB promoter.
Similar articles
-
The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis.Mol Microbiol. 1996 Jun;20(5):1043-55. doi: 10.1111/j.1365-2958.1996.tb02545.x. Mol Microbiol. 1996. PMID: 8809757
-
Regulation of transcription at the ndh promoter of Escherichia coli by FNR and novel factors.Mol Microbiol. 1994 May;12(3):433-44. doi: 10.1111/j.1365-2958.1994.tb01032.x. Mol Microbiol. 1994. PMID: 8065261
-
FNR-dependent repression of ndh gene expression requires two upstream FNR-binding sites.Microbiology (Reading). 1997 May;143 ( Pt 5):1521-1532. doi: 10.1099/00221287-143-5-1521. Microbiology (Reading). 1997. PMID: 9168602
-
FNR and its role in oxygen-regulated gene expression in Escherichia coli.FEMS Microbiol Rev. 1990 Aug;6(4):399-428. doi: 10.1111/j.1574-6968.1990.tb04109.x. FEMS Microbiol Rev. 1990. PMID: 2248796 Review.
-
Oxygen regulated gene expression in Escherichia coli: control of anaerobic respiration by the FNR protein.Antonie Van Leeuwenhoek. 1991 Feb;59(2):65-76. doi: 10.1007/BF00445650. Antonie Van Leeuwenhoek. 1991. PMID: 1854188 Review.
Cited by
-
Contributions of [4Fe-4S]-FNR and integration host factor to fnr transcriptional regulation.J Bacteriol. 2007 Apr;189(8):3036-43. doi: 10.1128/JB.00052-07. Epub 2007 Feb 9. J Bacteriol. 2007. PMID: 17293415 Free PMC article.
-
Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expression of MarA.J Bacteriol. 2000 Jun;182(12):3467-74. doi: 10.1128/JB.182.12.3467-3474.2000. J Bacteriol. 2000. PMID: 10852879 Free PMC article.
-
Large-scale 13C-flux analysis reveals distinct transcriptional control of respiratory and fermentative metabolism in Escherichia coli.Mol Syst Biol. 2011 Mar 29;7:477. doi: 10.1038/msb.2011.9. Mol Syst Biol. 2011. PMID: 21451587 Free PMC article.
-
PdhR (pyruvate dehydrogenase complex regulator) controls the respiratory electron transport system in Escherichia coli.J Bacteriol. 2007 Aug;189(15):5534-41. doi: 10.1128/JB.00229-07. Epub 2007 May 18. J Bacteriol. 2007. PMID: 17513468 Free PMC article.
-
New insights into type II NAD(P)H:quinone oxidoreductases.Microbiol Mol Biol Rev. 2004 Dec;68(4):603-16. doi: 10.1128/MMBR.68.4.603-616.2004. Microbiol Mol Biol Rev. 2004. PMID: 15590775 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
