Non-invasive measurement of endothelial function: effect on brachial artery dilatation of graded endothelial dependent and independent stimuli
- PMID: 9290397
- PMCID: PMC484859
- DOI: 10.1136/hrt.78.1.22
Non-invasive measurement of endothelial function: effect on brachial artery dilatation of graded endothelial dependent and independent stimuli
Abstract
Objective: To examine the relation between endothelial dependent and endothelial independent stimuli of varying intensity and measures of vascular function in the brachial artery of young healthy adults, to determine whether these responses are consistent and can be used to assess endothelial function.
Design and setting: High resolution ultrasound was used to measure brachial artery diameter at rest, after reactive hyperaemia induced by forearm cuff occlusion, and after sublingual isosorbide dinitrate (ISDN). Each subject was assigned to a random order of six cuff occlusion times (30 seconds, 1.5, 2.5, 3.5, 4.5, and 8 minutes) and six doses of ISDN (10, 20, 50, 100, 200, and 400 micrograms).
Subjects: Three males and three females mean age 31 years (range 25 to 43) with no known risk factors for cardiovascular disease.
Main outcome measures: Flow mediated, endothelial dependent dilatation was compared to duration of cuff occlusion, peak reactive hyperaemia, and duration of increased flow. Nitrate induced, endothelial independent dilatation was compared to dose of ISDN and change in flow.
Results: Vessel dilatation and duration of peak flow change increased with longer duration of cuff occlusion. After 4.5 minutes of occlusion, flow mediated dilatation was mean (SD) 96 (6)% of maximal response and did not increase significantly with longer occlusion times. No significant dilatation occurred after 10 or 20 micrograms of ISDN in any subject. With increasing doses up to 200 micrograms there was an increase in dilatation. Endothelial independent dilatation did not increase significantly with doses of ISDN above 200 micrograms.
Conclusions: Measures of arterial function vary with duration of blood flow occlusion and ISDN dose. Maximum arterial response was reached in all subjects after 4.5 minutes of blood flow occlusion or 200 micrograms of ISDN. Therefore, these variables produce consistent reproducible measures of endothelial function.
Similar articles
-
Handgrip exercise increases postocclusion hyperaemic brachial artery dilatation.Heart. 1999 Jul;82(1):93-5. doi: 10.1136/hrt.82.1.93. Heart. 1999. PMID: 10377317 Free PMC article.
-
Adding ischaemic hand exercise during occlusion of the brachial artery increases the flow-mediated vasodilation in ultrasound studies of endothelial function.Clin Physiol. 1999 Jul;19(4):279-83. doi: 10.1046/j.1365-2281.1999.00147.x. Clin Physiol. 1999. PMID: 10451786
-
Noninvasive assessment of endothelium-dependent flow-mediated dilation of the brachial artery.Vasc Med. 1997;2(2):87-92. doi: 10.1177/1358863X9700200203. Vasc Med. 1997. PMID: 9546961
-
New methods to evaluate endothelial function: Non-invasive method of evaluating endothelial function in humans.J Pharmacol Sci. 2003 Dec;93(4):405-8. doi: 10.1254/jphs.93.405. J Pharmacol Sci. 2003. PMID: 14737009 Review.
-
Special article: non-invasive measurement of endothelial function.Clin Exp Pharmacol Physiol. 1998 Jul-Aug;25(7-8):640-3. doi: 10.1111/j.1440-1681.1998.tb02269.x. Clin Exp Pharmacol Physiol. 1998. PMID: 9673445 Review.
Cited by
-
Noninvasive Studies of Coronary and Peripheral Arterial Blood-flow.Curr Atheroscler Rep. 2002 Sep;4(5):381-5. doi: 10.1007/s11883-002-0076-5. Curr Atheroscler Rep. 2002. PMID: 12162938 Review.
-
Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test.J Vis Exp. 2016 Apr 27;(110):54011. doi: 10.3791/54011. J Vis Exp. 2016. PMID: 27167596 Free PMC article.
-
Associations of sex, age and adiposity in endothelium-independent dilation in children.Physiol Meas. 2018 Apr 20;39(4):045002. doi: 10.1088/1361-6579/aab4cf. Physiol Meas. 2018. PMID: 29513264 Free PMC article.
-
Kinetic differences between macro- and microvascular measures of reactive hyperemia.J Appl Physiol (1985). 2020 Nov 1;129(5):1183-1192. doi: 10.1152/japplphysiol.00481.2020. Epub 2020 Sep 17. J Appl Physiol (1985). 2020. PMID: 32940560 Free PMC article.
-
Arterial stiffness or endothelial dysfunction as a surrogate marker of vascular risk.Can J Cardiol. 2006 Feb;22 Suppl B(Suppl B):72B-80B. doi: 10.1016/s0828-282x(06)70990-4. Can J Cardiol. 2006. PMID: 16498516 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources