Reperfusion-induced leukocyte adhesion and vascular protein leakage in normal and hypercholesterolemic rats
- PMID: 9277503
- DOI: 10.1152/ajpheart.1997.273.2.H854
Reperfusion-induced leukocyte adhesion and vascular protein leakage in normal and hypercholesterolemic rats
Abstract
The objective of this study was to define the influence of hypercholesterolemia on ischemia-reperfusion (I/R)-induced leukocyte-endothelial cell adhesion and albumin leakage in rat mesenteric venules. The microvascular alterations normally elicited by I/R (leukocyte adherence and emigration, albumin leakage, and platelet aggregation) were more pronounced in hypercholesterolemic rats (compared with control rats). Monoclonal antibodies against the adhesion glycoproteins CD11/CD18 and intercellular adhesion molecule-1 attenuated the I/R-induced leukocyte adherence and emigration and albumin leakage. Leukocyte adherence, but not albumin leakage, was diminished in animals pretreated with a P-selectin-specific antibody. Platelet aggregation was reduced by antibodies directed against either P-selectin, CD18, or intercellular adhesion molecule-1, as well as a GPIIb-IIIa antagonist. These results indicate that the enhanced reperfusion-induced albumin leakage in hypercholesterolemic rats is dependent on leukocyte-endothelial cell adhesion. Furthermore, P-selectin- and CD11/CD18-dependent heterotypic and GPIIb-IIIa-mediated homotypic platelet aggregation appear to influence the extravasation of both leukocytes and albumin in postischemic venules of hypercholesterolemic rats.
Similar articles
-
Molecular determinants of reperfusion-induced leukocyte adhesion and vascular protein leakage.Circ Res. 1994 Feb;74(2):336-43. doi: 10.1161/01.res.74.2.336. Circ Res. 1994. PMID: 7507416
-
Clostridium difficile toxin A-induced microvascular dysfunction. Role of histamine.J Clin Invest. 1994 Nov;94(5):1919-26. doi: 10.1172/JCI117542. J Clin Invest. 1994. PMID: 7962537 Free PMC article.
-
Ischemia/reperfusion-induced microvascular dysfunction: role of oxidants and lipid mediators.Am J Physiol. 1997 Jun;272(6 Pt 2):H2976-82. doi: 10.1152/ajpheart.1997.272.6.H2976. Am J Physiol. 1997. PMID: 9227576
-
Leukocyte--endothelial cell adhesion induced by ischemia and reperfusion.Can J Physiol Pharmacol. 1993 Jan;71(1):67-75. doi: 10.1139/y93-011. Can J Physiol Pharmacol. 1993. PMID: 8513435 Review.
-
Monoclonal antibodies to leukocyte and endothelial adhesion molecules attenuate ischemia-reperfusion injury.Behring Inst Mitt. 1993 Aug;(92):229-37. Behring Inst Mitt. 1993. PMID: 8250813 Review.
Cited by
-
The role of DNA sensing and innate immune receptor TLR9 in otitis media.Innate Immun. 2012 Feb;18(1):3-13. doi: 10.1177/1753425910393539. Epub 2011 Jan 14. Innate Immun. 2012. PMID: 21239460 Free PMC article.
-
The microcirculation in adipose tissue inflammation.Rev Endocr Metab Disord. 2013 Mar;14(1):69-76. doi: 10.1007/s11154-013-9236-x. Rev Endocr Metab Disord. 2013. PMID: 23378133 Free PMC article. Review.
-
Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage.J Neurosci Res. 2008 Nov 1;86(14):3240-5. doi: 10.1002/jnr.21762. J Neurosci Res. 2008. PMID: 18615643 Free PMC article.
-
Role of blood cells in ischaemia-reperfusion induced endothelial barrier failure.Cardiovasc Res. 2010 Jul 15;87(2):291-9. doi: 10.1093/cvr/cvq090. Epub 2010 Mar 17. Cardiovasc Res. 2010. PMID: 20299333 Free PMC article. Review.
-
Platelets, acting in part via P-selectin, mediate cytomegalovirus-induced microvascular dysfunction.Am J Physiol Heart Circ Physiol. 2014 Dec 15;307(12):H1745-53. doi: 10.1152/ajpheart.00201.2014. Epub 2014 Oct 17. Am J Physiol Heart Circ Physiol. 2014. PMID: 25326535 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
