Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 Sep;35(9):2370-4.
doi: 10.1128/jcm.35.9.2370-2374.1997.

Identification of a Klebsiella pneumoniae strain associated with nosocomial urinary tract infection

K S Kil  1 , R O Darouiche, R A Hull, M D Mansouri, D M Musher
Affiliations

Identification of a Klebsiella pneumoniae strain associated with nosocomial urinary tract infection

K S Kil et al. J Clin Microbiol. 1997 Sep.

Abstract

To differentiate between relapse of infection and reinfection of the urinary tract due to Klebsiella pneumoniae, 33 K. pneumoniae isolates collected from 20 patients with spinal cord injury (SCI) over 2 years were typed by genomic fingerprinting by repetitive-element PCR. Clinical isolates obtained from the same patients with recurrent episodes of urinary tract infection (UTI) revealed identical genomic fingerprints indicating relapse of UTI due to K. pneumoniae, despite appropriate antibiotic therapy. Seventeen isolates obtained from 8 of the 20 SCI patients shared a common genotype, termed RD6. Among non-SCI patients residing in other nursing units, the RD6 genotype was found in 5 of 10 patients with K. pneumoniae UTI but in only 1 of 20 patients with K. pneumoniae infection that did not involve the urinary tract, suggesting a strong association of this genotype with UTI. All RD6 isolates exhibited strong adherence (> or =50 adherent bacteria per cell) to HEp-2 cells, whereas other K. pneumoniae isolates generally did not adhere to or adhered very weakly to HEp-2 cells (< or =5 adherent bacteria per cell). Adherence was inhibited either by 4% D-mannose or by anti-type 1 fimbrial rabbit serum. These results suggest that the capacity of K. pneumoniae RD6 isolates to cause UTI may be mediated by its striking adherence to mammalian cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. N Engl J Med. 1966 Jul 14;275(2):70-3 - PubMed
    1. J Gen Microbiol. 1959 Aug;21:271-86 - PubMed
    1. Infect Immun. 1985 May;48(2):275-9 - PubMed
    1. Diagn Microbiol Infect Dis. 1987 Aug;7(4):229-35 - PubMed
    1. Infect Immun. 1988 Nov;56(11):3011-3 - PubMed

MeSH terms