Targeted complementation of MHC class II deficiency by intrathymic delivery of recombinant adenoviruses
- PMID: 9252125
- DOI: 10.1016/s1074-7613(00)80515-4
Targeted complementation of MHC class II deficiency by intrathymic delivery of recombinant adenoviruses
Abstract
De novo differentiation of CD4+ T cells was provoked in mice lacking major histocompatibility complex (MHC) class II molecules by intrathymic injection of adenovirus vectors carrying class II genes. This permits a new approach to questions concerning the dynamics of CD4+ T cell compartments in the thymus and peripheral lymphoid organs. Here two issues are explored. First, we show that mature CD4+ CD8- cells reside in the thymus for a protracted period before emigrating to the periphery, highlighting the potential importance of, and our ignorance of, the postselection maturation period. Second, we demonstrate that the survival of CD4+ cells in peripheral lymphoid organs is markedly curtailed when class II molecules are absent and is not further reduced in the absence of both class II and class I molecules, raising the possibility that MHC-mediated selection may continue in the periphery.
Similar articles
-
Positive selection of thymocytes induced by gene transfer: MHC class II-mediated selection of CD8 lineage cells.Int Immunol. 1999 Oct;11(10):1595-600. doi: 10.1093/intimm/11.10.1595. Int Immunol. 1999. PMID: 10508177
-
CD4+ T cells mature in the absence of MHC class I and class II expression in Ly-6A.2 transgenic mice.J Immunol. 1998 Jul 1;161(1):175-82. J Immunol. 1998. PMID: 9647222
-
Cross-positive selection of thymocytes expressing a single TCR by multiple major histocompatibility complex molecules of both classes: implications for CD4+ versus CD8+ lineage commitment.J Immunol. 2006 Feb 1;176(3):1628-36. doi: 10.4049/jimmunol.176.3.1628. J Immunol. 2006. PMID: 16424192
-
The role of CD4 in regulating homeostasis of T helper cells.Immunol Res. 2002;25(2):115-30. doi: 10.1385/IR:25:2:115. Immunol Res. 2002. PMID: 11999166 Review.
-
MHC and T cell development.Rev Immunogenet. 1999;1(1):91-104. Rev Immunogenet. 1999. PMID: 11256575 Review.
Cited by
-
The final maturation of at least some single-positive CD4(hi) thymocytes does not require T cell receptor-major histocompatibility complex contact.J Exp Med. 1999 Sep 20;190(6):757-64. doi: 10.1084/jem.190.6.757. J Exp Med. 1999. PMID: 10499914 Free PMC article.
-
gammadeltaTCR ligands and lineage commitment.Semin Immunol. 2010 Aug;22(4):214-21. doi: 10.1016/j.smim.2010.04.001. Epub 2010 May 5. Semin Immunol. 2010. PMID: 20447836 Free PMC article. Review.
-
A T cell receptor-specific blockade of positive selection.J Exp Med. 1999 Jan 4;189(1):13-24. doi: 10.1084/jem.189.1.13. J Exp Med. 1999. PMID: 9874560 Free PMC article.
-
Quantitative analysis of T cell homeostatic proliferation.Cell Immunol. 2007 Nov-Dec;250(1-2):40-54. doi: 10.1016/j.cellimm.2008.01.003. Epub 2008 Mar 3. Cell Immunol. 2007. PMID: 18313651 Free PMC article.
-
Asymmetric thymocyte death underlies the CD4:CD8 T-cell ratio in the adaptive immune system.Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):E2905-14. doi: 10.1073/pnas.1304859110. Epub 2013 Jul 15. Proc Natl Acad Sci U S A. 2013. PMID: 23858460 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
