doi: 10.1006/viro.1997.8597.
Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line
Affiliations
- PMID: 9217065
- DOI: 10.1006/viro.1997.8597
Item in Clipboard
Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line
Virology.
.
Free article
Abstract
The human-viral junctions of integrated adenovirus type 5 (Ad5) DNA in 293 cells have been cloned and sequenced. The Ad5 sequences extend from nucleotides (nt) 1 to 4344 and are located in the pregnancy-specific beta-1-glycoprotein 4 (PSG 4) gene. This maps the insertion of Ad5 DNA to human chromosome 19 (19q13.2). The Ad5 sequences are represented as a single collinear insertion of viral DNA with no rearrangements. There were 19 bp of PSG4 DNA deleted at the site of insertion and an extra 3 nt (GTC) at the left viral/cellular junction. A short patch of homology between viral DNA and PSG4 DNA (6 nt with 1 mismatch) was present at the right junction.
Similar articles
-
Clonal origin of adenovirus type 12-induced hamster tumors: nonspecific chromosomal integration sites of viral DNA.Cancer Res. 1997 Jul 15;57(14):3001-9. Cancer Res. 1997. PMID: 9230215
-
Effect of methyl methanesulfonate on type 5 adenovirus DNA integration and the phenotypic properties of cold-sensitive type 5 adenovirus-transformed cloned rat embryo fibroblast cells.Cancer Res. 1988 Jun 1;48(11):3050-7. Cancer Res. 1988. PMID: 3365695
-
[Cloning and sequencing of adenovirus type 7 vaccine strain 76.5-87 mu fragment].Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1998 Sep;12(3):201-6. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1998. PMID: 12526316 Chinese.
-
Spontaneous occurrence of early region 1A reiteration mutants of type 5 adenovirus in persistently infected human T-lymphocytes.Virology. 1997 Apr 14;230(2):281-91. doi: 10.1006/viro.1997.8482. Virology. 1997. PMID: 9143284
-
Uptake of foreign DNA by mammalian cells via the gastrointestinal tract in mice: methylation of foreign DNA--a cellular defense mechanism.Curr Top Microbiol Immunol. 1995;197:209-24. doi: 10.1007/978-3-642-79145-1_14. Curr Top Microbiol Immunol. 1995. PMID: 7493494 Review. No abstract available.
Cited by
-
Identification of an ADAM17 cleavage region in human CD16 (FcγRIII) and the engineering of a non-cleavable version of the receptor in NK cells.PLoS One. 2015 Mar 27;10(3):e0121788. doi: 10.1371/journal.pone.0121788. eCollection 2015. PLoS One. 2015. PMID: 25816339 Free PMC article.
-
A Method to Generate and Rescue Recombinant Adenovirus Devoid of Replication-Competent Particles in Animal-Origin-Free Culture Medium.Viruses. 2023 Oct 25;15(11):2152. doi: 10.3390/v15112152. Viruses. 2023. PMID: 38005830 Free PMC article.
-
293 cells express both epithelial as well as mesenchymal cell adhesion molecules.Int J Mol Med. 2016 Jun;37(6):1521-7. doi: 10.3892/ijmm.2016.2568. Epub 2016 Apr 18. Int J Mol Med. 2016. PMID: 27121032 Free PMC article.
-
Development and characterization of bovine x human hybrid cell lines that efficiently support the replication of both wild-type bovine and human adenoviruses and those with E1 deleted.J Virol. 2002 Jun;76(12):5882-92. doi: 10.1128/jvi.76.12.5882-5892.2002. J Virol. 2002. PMID: 12021321 Free PMC article.
-
Respiratory viral infection: a potential "missing link" in the pathogenesis of COPD.Eur Respir Rev. 2019 Mar 14;28(151):180063. doi: 10.1183/16000617.0063-2018. Print 2019 Mar 31. Eur Respir Rev. 2019. PMID: 30872396 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
