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. 1997 Jun;17(6):3194-201.
doi: 10.1128/MCB.17.6.3194.

The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo

R J Buckanovich  1 , R B Darnell
Affiliations

The neuronal RNA binding protein Nova-1 recognizes specific RNA targets in vitro and in vivo

R J Buckanovich et al. Mol Cell Biol. 1997 Jun.

Abstract

Nova-1, an autoantigen in paraneoplastic opsoclonus myoclonus ataxia (POMA), a disorder associated with breast cancer and motor dysfunction, is a neuron-specific nuclear RNA binding protein. We have identified in vivo Nova-1 RNA ligands by combining affinity-elution-based RNA selection with protein-RNA immunoprecipitation. Starting with a pool of approximately 10(15) random 52-mer RNAs, we identified long stem-loop RNA ligands that bind to Nova-1 with high affinity (Kd of approximately 2 nM). The loop region of these RNAs harbors a approximately 15-bp pyrimidine-rich element [UCAU(N)(0-2)]3 which is essential for Nova-1 binding. Mutagenesis studies defined the third KH domain of Nova-1 and the [UCAU(N)(0-2)]3 element as necessary for in vitro binding. Consensus [UCAU (N)(0-2)], elements were identified in two neuronal pre-mRNAs, one encoding the inhibitory glycine receptor alpha2 (GlyR alpha2) and a second encoding Nova-1 itself. Nova-1 protein binds these RNAs with high affinity and specificity in vitro, and this binding can be blocked by POMA antisera. Moreover, both Nova-1 and GlyR alpha2 pre-mRNAs specifically coimmunoprecipitated with Nova-1 protein from brain extracts. Thus, Nova-1 functions as a sequence-specific nuclear RNA binding protein in vivo; disruption of the specific interaction between Nova-1 and GlyR alpha2 pre-mRNA may underlie the motor dysfunction seen in POMA.

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References

    1. EMBO J. 1994 Mar 1;13(5):1197-204 - PubMed
    1. Nat Genet. 1993 Dec;5(4):351-8 - PubMed
    1. Genes Dev. 1994 Jan;8(2):211-20 - PubMed
    1. Mol Cell Biol. 1993 Jan;13(1):703-10 - PubMed
    1. Nature. 1993 Mar 11;362(6416):171-5 - PubMed

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