doi: 10.1006/jmbi.1997.0955.
Study of plasmid replication in Escherichia coli with a combination of 2D gel electrophoresis and electron microscopy
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- PMID: 9149135
- DOI: 10.1006/jmbi.1997.0955
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Study of plasmid replication in Escherichia coli with a combination of 2D gel electrophoresis and electron microscopy
J Mol Biol.
.
Abstract
We studied theta-mode DNA replication in p15A-based Escherichia coli plasmids by analyzing their replication intermediates using a combination of neutral agarose 2D gel electrophoresis and electron microscopy. Our analysis: (1) confirms the original assignment of various features of the 2D gel pattern; (2) shows that while one replication fork progresses around the plasmid DNA, the other is immobile, as if the replication were unidirectional; and (3) reveals that termination often occurs at a location away from the replication origin, suggesting that the replication of our plasmids is, in fact, bidirectional, the two forks being active at different times.
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