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. 1997 Apr 1;16(7):1501-7.
doi: 10.1093/emboj/16.7.1501.

Substrate specificity of the DnaK chaperone determined by screening cellulose-bound peptide libraries

S Rüdiger  1 , L Germeroth, J Schneider-Mergener, B Bukau
Affiliations

Substrate specificity of the DnaK chaperone determined by screening cellulose-bound peptide libraries

S Rüdiger et al. EMBO J. .

Abstract

Hsp70 chaperones assist protein folding by ATP-dependent association with linear peptide segments of a large variety of folding intermediates. The molecular basis for this ability to differentiate between native and non-native conformers was investigated for the DnaK homolog of Escherichia coli. We identified binding sites and the recognition motif in substrates by screening 4360 cellulose-bound peptides scanning the sequences of 37 biologically relevant proteins. DnaK binding sites in protein sequences occurred statistically every 36 residues. In the folded proteins these sites are mostly buried and in the majority found in beta-sheet elements. The binding motif consists of a hydrophobic core of four to five residues enriched particularly in Leu, but also in Ile, Val, Phe and Tyr, and two flanking regions enriched in basic residues. Acidic residues are excluded from the core and disfavored in flanking regions. The energetic contribution of all 20 amino acids for DnaK binding was determined. On the basis of these data an algorithm was established that predicts DnaK binding sites in protein sequences with high accuracy.

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