doi: 10.1016/0378-1119(96)00158-8.
Analysis of pseudorabies virus genes by cDNA sequencing
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- PMID: 8917106
- DOI: 10.1016/0378-1119(96)00158-8
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Analysis of pseudorabies virus genes by cDNA sequencing
Gene.
.
Abstract
Cloning and sequencing of cDNA could provide a complementary approach to functional analysis of the pseudorabies virus (PrV) genome. Using colony hybridization, Southern hybridization, and DNA sequencing, four species of PrV-specific cDNA were identified. Among these four species of PrV-specific cDNA, three unidentified genes, UL26, UL29, and UL31, were mapped and a novel gI-11K bicistronic cDNA was confirmed. Thus, analysis of PrV-specific transcripts provided a way for identifying genes and a foundation to further study the roles of these transcripts in PrV infection.
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