The interaction of DNA with bacteriophage phi 29 connector: a study by AFM and TEM
- PMID: 8812997
- DOI: 10.1006/jsbi.1996.0056
The interaction of DNA with bacteriophage phi 29 connector: a study by AFM and TEM
Abstract
The connector of bacteriophage phi 29 is involved in DNA packaging during viral morphogenesis and we have studied its in vitro binding to DNA using either linear or circular DNA. The protein-DNA complexes have been analyzed by transmission electron microscopy (TEM) and by atomic force microscopy (AFM) of samples directly deposited on mica. TEM showed the presence of a specific binding due to the interaction of the protein with the free ends of the DNA. The study of these samples by AFM showed two major types of morphologies: The interaction of the connector with circular DNA revealed that the strands of DNA that enter and exit the protein complex form an angle with a mean value of 132 degrees. Nevertheless, when the connector was incubated with linear DNA (and later circularized), there was an additional bend angle of about 168 degrees. Further morphological analysis of the latter samples by AFM revealed a structure of the protein-DNA complex consistent with the DNA traversing the connector, probably through the inner channel. On the other hand, images from the samples obtained by incubation of the connector with circular DNa were consistent with an interaction of the DNA with the outer side of the connector.
Similar articles
-
The bacteriophage phi29 head-tail connector imaged at high resolution with the atomic force microscope in buffer solution.EMBO J. 1997 May 15;16(10):2547-53. doi: 10.1093/emboj/16.10.2547. EMBO J. 1997. PMID: 9184202 Free PMC article.
-
Topology of the components of the DNA packaging machinery in the phage phi29 prohead.J Mol Biol. 2000 May 19;298(5):807-15. doi: 10.1006/jmbi.2000.3712. J Mol Biol. 2000. PMID: 10801350
-
Domain architecture of the bacteriophage phi29 connector protein.J Mol Biol. 1999 May 21;288(5):899-909. doi: 10.1006/jmbi.1999.2731. J Mol Biol. 1999. PMID: 10329188
-
Picture story. A DNA wormhole.Nat Struct Biol. 2001 Jan;8(1):20. doi: 10.1038/83000. Nat Struct Biol. 2001. PMID: 11135663 No abstract available.
-
Molecular structure determination by high resolution electron microscopy.Annu Rev Biophys Bioeng. 1979;8:129-44. doi: 10.1146/annurev.bb.08.060179.001021. Annu Rev Biophys Bioeng. 1979. PMID: 383000 Review. No abstract available.
Cited by
-
DNA bending and unbending by MutS govern mismatch recognition and specificity.Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14822-7. doi: 10.1073/pnas.2433654100. Epub 2003 Nov 21. Proc Natl Acad Sci U S A. 2003. PMID: 14634210 Free PMC article.
-
Single-particle virology.Biophys Rev. 2020 Oct;12(5):1141-1154. doi: 10.1007/s12551-020-00747-9. Epub 2020 Sep 3. Biophys Rev. 2020. PMID: 32880826 Free PMC article. Review.
-
The bacteriophage phi29 head-tail connector imaged at high resolution with the atomic force microscope in buffer solution.EMBO J. 1997 May 15;16(10):2547-53. doi: 10.1093/emboj/16.10.2547. EMBO J. 1997. PMID: 9184202 Free PMC article.
-
High-resolution imaging of antibodies by tapping-mode atomic force microscopy: attractive and repulsive tip-sample interaction regimes.Biophys J. 2000 Mar;78(3):1599-605. doi: 10.1016/S0006-3495(00)76712-9. Biophys J. 2000. PMID: 10692344 Free PMC article.
-
Alterations of the portal protein, gpB, of bacteriophage lambda suppress mutations in cosQ, the site required for termination of DNA packaging.Genetics. 2002 May;161(1):21-31. doi: 10.1093/genetics/161.1.21. Genetics. 2002. PMID: 12019220 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
