Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain protein Shb
- PMID: 8806685
Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain protein Shb
Abstract
To understand the role of the Src homology 2 (SH2) domain protein Shb in the signal transduction of tyrosine kinase receptor, NIH3T3 cells were transfected with a DNA construct expressing the Shb cDNA (NIHSHB cells). The NIHSHB cells expressed elevated levels of proteins with the estimated molecular weights of 77, 66 and 55 kDa as determined by immunoblotting. In contrast to the control cells, the NIHSHB cells failed to increase in cell number in the presence of 1% serum. This effect was largely due to apoptosis, since staining of pyknotic nuclei was observed using the terminal transferase labeling method. The NIHSHB cells displayed similar levels of c-myc mRNA and decreased contents of the p53 protein after culture in 1% serum compared with control cells. The addition of platelet-derived growth factor (PDGF-BB) restored the growth of the NIHSHB cells, whereas insulin-like growth factor-1 (IGF-1) failed to affect the proliferation of Shb overexpressing cells in 1% serum. We conclude that Shb overexpression is associated with cell degeneration under certain conditions, and that Shb could transduce apoptotic signals from tyrosine kinase receptors.
Similar articles
-
Modulation of Src homology 3 proteins by the proline-rich adaptor protein Shb.Exp Cell Res. 1997 Mar 15;231(2):269-75. doi: 10.1006/excr.1996.3471. Exp Cell Res. 1997. PMID: 9087167
-
Shb is a ubiquitously expressed Src homology 2 protein.Oncogene. 1994 Jan;9(1):19-27. Oncogene. 1994. PMID: 8302579
-
Shf, a Shb-like adapter protein, is involved in PDGF-alpha-receptor regulation of apoptosis.Biochem Biophys Res Commun. 2000 Nov 30;278(3):537-43. doi: 10.1006/bbrc.2000.3847. Biochem Biophys Res Commun. 2000. PMID: 11095946
-
Reduced tumor growth in vivo and increased c-Abl activity in PC3 prostate cancer cells overexpressing the Shb adapter protein.BMC Cancer. 2007 Aug 15;7:161. doi: 10.1186/1471-2407-7-161. BMC Cancer. 2007. PMID: 17697368 Free PMC article.
-
Role of tyrosine kinase signaling for beta-cell replication and survival.Ups J Med Sci. 2000;105(2):7-15. doi: 10.1517/03009734000000052. Ups J Med Sci. 2000. PMID: 11095102 Review.
Cited by
-
The Shb adaptor protein binds to tyrosine 766 in the FGFR-1 and regulates the Ras/MEK/MAPK pathway via FRS2 phosphorylation in endothelial cells.Mol Biol Cell. 2002 Aug;13(8):2881-93. doi: 10.1091/mbc.e02-02-0103. Mol Biol Cell. 2002. PMID: 12181353 Free PMC article.
-
Shb deficient mice display an augmented TH2 response in peripheral CD4+ T cells.BMC Immunol. 2011 Jan 11;12:3. doi: 10.1186/1471-2172-12-3. BMC Immunol. 2011. PMID: 21223549 Free PMC article.
-
Angiostatin induces endothelial cell apoptosis and activation of focal adhesion kinase independently of the integrin-binding motif RGD.Proc Natl Acad Sci U S A. 1998 May 12;95(10):5579-83. doi: 10.1073/pnas.95.10.5579. Proc Natl Acad Sci U S A. 1998. PMID: 9576925 Free PMC article.
-
Molecular mechanisms of OLIG2 transcription factor in brain cancer.Oncotarget. 2016 Aug 16;7(33):53074-53101. doi: 10.18632/oncotarget.10628. Oncotarget. 2016. PMID: 27447975 Free PMC article.
-
Transgenic mice expressing Shb adaptor protein under the control of rat insulin promoter exhibit altered viability of pancreatic islet cells.Mol Med. 1999 Mar;5(3):169-80. Mol Med. 1999. PMID: 10404514 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases
Research Materials
Miscellaneous