Altered expression of bcl-2 and bax mRNA in amyotrophic lateral sclerosis spinal cord motor neurons
- PMID: 8797527
- DOI: 10.1002/ana.410400307
Altered expression of bcl-2 and bax mRNA in amyotrophic lateral sclerosis spinal cord motor neurons
Abstract
One of the primary neurodegenerative events occurring in amyotrophic lateral sclerosis (ALS) is the selective loss of spinal cord alpha motor neurons. To study the potential role of apoptosis in the degeneration of these motor neurons, in situ hybridization was used to measure the expression of two apoptotic cell death genes, bcl-2 and bax, in control and ALS lumbar spinal cord sections. The strongest hybridization signal for bcl-2 mRNA in neurological and nonneurological control spinal cords was found primarily in lamina IX alpha motor neurons, while a weaker hybridization signal was found in neurons of Clarke's nucleus and the proper sensory nucleus of the dorsal horn. Surviving lamina IX motor neurons in ALS spinal cord sections also expressed bcl-2 mRNA, but at levels that were significantly and selectively decreased (4.7-fold) compared with controls. bax mRNA hybridization signal was detected in several cells throughout the gray matter in control and ALS lumbar spinal cord, but was significantly and selectively increased (2.8-fold) in ALS motor neurons. Given the proposed interactive roles of these genes in apoptosis, the present findings favor a scenario in which this mode of cell death would contribute to spinal cord motor neuron degeneration in ALS.
Similar articles
-
FAC1 expression and localization in motor neurons of developing, adult, and amyotrophic lateral sclerosis spinal cord.Exp Neurol. 1997 Jul;146(1):17-24. doi: 10.1006/exnr.1997.6508. Exp Neurol. 1997. PMID: 9225734
-
Beta-amyloid 42 accumulation in the lumbar spinal cord motor neurons of amyotrophic lateral sclerosis patients.Neurobiol Dis. 2005 Jun-Jul;19(1-2):340-7. doi: 10.1016/j.nbd.2005.01.012. Neurobiol Dis. 2005. PMID: 15837590
-
Persistent cleavage and nuclear translocation of apoptosis-inducing factor in motor neurons in the spinal cord of sporadic amyotrophic lateral sclerosis patients.Acta Neuropathol. 2009 Dec;118(6):755-62. doi: 10.1007/s00401-009-0580-6. Acta Neuropathol. 2009. PMID: 19669652
-
[A clinicopathological study of familial amyotrophic lateral sclerosis with special reference to the mode of motoneuron loss in the spinal ventral horn].Rinsho Shinkeigaku. 1991 Sep;31(9):960-9. Rinsho Shinkeigaku. 1991. PMID: 1769159 Review. Japanese.
-
AMPA receptor-mediated neuronal death in sporadic ALS.Neuropathology. 2010 Apr;30(2):182-8. doi: 10.1111/j.1440-1789.2009.01090.x. Epub 2010 Jan 19. Neuropathology. 2010. PMID: 20102521 Review.
Cited by
-
Molecular mechanisms of cell death in neurological diseases.Cell Death Differ. 2021 Jul;28(7):2029-2044. doi: 10.1038/s41418-021-00814-y. Epub 2021 Jun 7. Cell Death Differ. 2021. PMID: 34099897 Free PMC article. Review.
-
Cell cycle regulators in the neuronal death pathway of amyotrophic lateral sclerosis caused by mutant superoxide dismutase 1.J Neurosci. 2003 Mar 15;23(6):2131-40. doi: 10.1523/JNEUROSCI.23-06-02131.2003. J Neurosci. 2003. PMID: 12657672 Free PMC article.
-
Reovirus infection and tissue injury in the mouse central nervous system are associated with apoptosis.J Virol. 1997 Mar;71(3):2100-6. doi: 10.1128/JVI.71.3.2100-2106.1997. J Virol. 1997. PMID: 9032342 Free PMC article.
-
Alterations in G(1) to S phase cell-cycle regulators during amyotrophic lateral sclerosis.Am J Pathol. 2003 Mar;162(3):823-35. doi: 10.1016/S0002-9440(10)63879-5. Am J Pathol. 2003. PMID: 12598317 Free PMC article.
-
Stable over-expression of the 2-oxoglutarate carrier enhances neuronal cell resistance to oxidative stress via Bcl-2-dependent mitochondrial GSH transport.J Neurochem. 2014 Jul;130(1):75-86. doi: 10.1111/jnc.12709. Epub 2014 Apr 2. J Neurochem. 2014. PMID: 24606213 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous
