Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Sep 3;93(18):9553-8.
doi: 10.1073/pnas.93.18.9553.

Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria

T J Evans  1 , L D Buttery, A Carpenter, D R Springall, J M Polak, J Cohen
Affiliations

Cytokine-treated human neutrophils contain inducible nitric oxide synthase that produces nitration of ingested bacteria

T J Evans et al. Proc Natl Acad Sci U S A. .

Abstract

Although the production of NO within rodent phagocytes is well-characterized, its production and function within human phagocytes are less clear. We show here that neutrophils within human buffy coat preparations stimulated with a mixture of interleukin 1, tumor necrosis factor alpha, and interferon gamma contain inducible NO synthase mRNA and protein, one of the enzymes responsible for NO production. The protein colocalizes with myeloperoxidase within neutrophil primary granules. Using an inhibitor of NO synthase, L-N-monomethyl arginine, we show that activity of this enzyme is required for the formation of nitrotyrosine around phagocytosed bacteria, most likely through the intermediate production of peroxynitrite, a reaction product of NO and superoxide anions.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1988 Nov 24;336(6197):385-8 - PubMed
    1. Methods Enzymol. 1996;269:210-18 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6328-32 - PubMed
    1. Biochem J. 1989 Jul 1;261(1):293-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1990 Feb;87(4):1620-4 - PubMed

Publication types

MeSH terms