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. 1996 Aug;70(8):5695-700.
doi: 10.1128/JVI.70.8.5695-5700.1996.

Fine mapping and characterization of the Rous sarcoma virus Pr76gag late assembly domain

Y Xiang  1 , C E Cameron, J W Wills, J Leis
Affiliations

Fine mapping and characterization of the Rous sarcoma virus Pr76gag late assembly domain

Y Xiang et al. J Virol. 1996 Aug.

Abstract

The p2 region of the Rous sarcoma virus (RSV) Gag polyprotein contains an assembly domain, which is required late in replication for efficient budding of virus-like particles from cells (J. W. Wills, C. E. Cameron, C. B. Wilson, Y. Xiang, R. P. Bennett, and J. Leis, J. Virol. 68:6605-6618, 1994). This domain, referred to as the L domain, was previously mapped to the 11 amino acids of p2b. Through the analysis of a series of deletion and substitution mutations, the L domain has now been fine mapped to a highly conserved amino acid sequence, PPPPYV of p2b. Sequences flanking PPPPYV motif can be deleted without any effect on budding. Defects caused by L-domain deletions can be rescued by placing a wild-type copy of the sequence at several other positions in RSV Gag. A proline-rich P(S/T)APP motif is found in many retroviral Gag polyproteins; the motif found in the p6 region of human immunodeficiency virus type 1 has been implicated in late functions of the virus. Substitution of the RSV L domain with this motif in a 10-amino-acid sequence derived from visna leukemia virus results in wild-type release of virus particles from cells. In contrast, the slightly different sequences from Gibbon ape leukemia virus, Moloney leukemia virus, PSAPP alone, or a proline-rich SH3 binding sequence do not efficiently rescue RSV L-domain mutations.

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References

    1. Eur J Biochem. 1989 Feb 1;179(2):415-22 - PubMed
    1. Methods Mol Biol. 1996;57:177-91 - PubMed
    1. J Virol. 1989 Oct;63(10):4331-43 - PubMed
    1. J Virol. 1990 Sep;64(9):4169-79 - PubMed
    1. J Virol. 1991 Jan;65(1):272-80 - PubMed

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